Influence of calcium on the early steps of rotavirus infection.
Influence of calcium on the early steps of rotavirus infection.
复制标题
钙对轮状病毒感染早期阶段的影响。
作者：
V. Pando;P. Iša;C. Arias;S. López
轮状病毒的结构及其复制周期的许多步骤取决于微环境中的钙浓度。在这项工作中，为了了解钙在感染早期阶段的作用，我们表征了增加培养液中钙浓度对轮状病毒感染性的影响。我们发现，细胞培养液中的钙浓度增加五倍会导致所有被测试的轮状病毒株的病毒滴度增加。这种二价离子的作用似乎主要在病毒颗粒上，而不是在细胞表面。对纯化的三层颗粒的本征荧光光谱分析表明，随着钙离子浓度的增加，色氨酸残基的环境发生了变化，提示病毒颗粒的构象变化可能是该离子影响病毒感染性的原因。
The structure of rotaviruses and many steps of their replication cycle depend on the concentration of calcium in the microenvironment. In this work, to learn about the role of calcium during the early steps of the infection, we characterized the effect of increasing the calcium concentration in the medium on the infectivity of rotaviruses. We found that a fivefold increase in the calcium concentration of the cell culture medium results in an increased viral titer in all rotavirus strains tested. The effect of this divalent ion seems to be mainly on the viral particle and not on the surface of the cell. Analysis of the intrinsic fluorescence spectra of purified triple-layered particles revealed that changes in the environment of tryptophan residues occurred as calcium concentration increased, suggesting that conformational changes in the viral particle might be responsible for the effect of this ion on the viral infectivity.
影响因子：
3.7
作者：
Dormitzer, PR;Greenberg, HB;Harrison, SC
通讯作者：
Harrison, SC
DOI：
10.1073/pnas.94.8.3960
发表时间：
1997-04-15
影响因子：
11.1
作者：
Dong, YJ;Zeng, CQY;Morris, AP
通讯作者：
Morris, AP
影响因子：
3.7
作者：
Wu, E;Fernandez, J;Nemerow, GR
通讯作者：
Nemerow, GR