Myocardial regeneration strategies using human embryonic stem cell-derived cardiomyocytes
Myocardial regeneration strategies using human embryonic stem cell-derived cardiomyocytes
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DOI：
10.1016/j.jconrel.2006.06.027
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发表时间：
2006-11-28
影响因子：
10.8
通讯作者：
Gepstein, Lior
中科院分区：
文献类型：
--
作者：
Capi, Oren;Gepstein, Lior
再生医学是一门新兴的生物医学学科，它利用干细胞生物学、分子生物学和组织工程领域的最新进展来衍生组织替代品，试图替代或改变患病器官的功能。由于成人心脏组织的再生能力有限，因此心脏是这些新兴技术的一个有吸引力的候选者。因此，心肌细胞替代疗法已成为恢复心肌机电功能的新型治疗范例。然而，由于人类心肌细胞的细胞来源匮乏，这种创新策略受到了严重阻碍。最近建立的人类胚胎干细胞（hESC）系可能为这一细胞来源问题提供可能的解决方案。这些独特的多能细胞系可以在培养物中以未分化状态繁殖，并诱导分化成所有三个胚层的细胞衍生物，包括心肌细胞。这篇综述将描述 hESC 系统、它们向心肌细胞的分化，以及这些心脏谱系衍生物的结构和功能特征。我们将讨论这种独特的分化系统在几个研究领域的潜在应用，特别强调充分利用其在心血管再生医学新兴领域的独特潜力所需的步骤。 (c) 2006 Elsevier B.V. 保留所有权利。
Regenerative medicine is a new biomedicine discipline that takes advantage of the recent advancements in the fields of stem cell biology, molecular biology, and tissue engineering to derive tissue substitutes, in an attempt to replace or modify the function of diseased organs. The heart represents an attractive candidate for these emerging technologies since adult cardiac tissue has limited regenerative capacity. Consequentially, myocardial cell replacement therapy has emerged as a novel therapeutic paradigm for restoration of the myocardial electromechanical function. This innovative strategy has been significantly hampered, however, by the paucity of cell sources for human cardiomyocytes. The recent establishment of the human embryonic stem cell (hESC) lines may provide a possible solution for this cell-sourcing problem. These unique pluripotent cell lines can be propagated in the undifferentiated state in culture and coaxed to differentiate into cell derivatives of all three germ layers, including cardiomyocytes. This review will describe the hESC system, their differentiation into cardiomyocytes, and the structural and functional characterization of these cardiac lineage derivatives. The potential applications of this unique differentiating system in several research areas will be discussed with special emphasis on the steps required to fully harness their unique potential in the emerging field of cardiovascular regenerative medicine. (c) 2006 Elsevier B.V. All rights reserved.