Production of active MMP7 in E. coli and its application for metalloproteinase inhibitors screening.
Production of active MMP7 in E. coli and its application for metalloproteinase inhibitors screening.
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DOI：
10.2174/092986610791112648
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发表时间：
2010-05
影响因子：
1.6
通讯作者：
H. Katsuno;R. Shirakawa;K. Miyazaki;Y. Ozeki;H. Yasumitsu
中科院分区：
文献类型：
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作者：
H. Katsuno;R. Shirakawa;K. Miyazaki;Y. Ozeki;H. Yasumitsu
MMP-7是最小的金属蛋白酶。近年来发现，它在血清中的不受调节的活性和存在与危及生命的疾病如心脏病和多种癌症密切相关。蛋白质的生产被认为是有用的，其表征和抗体的产生。尽管已经进行了许多细菌表达的尝试，但它们被回收为不溶性和无活性的蛋白质。本研究将其可溶性表达、一步纯化并转化为活性蛋白酶后，应用于人癌细胞条件培养液中分泌型金属蛋白酶抑制剂的筛选。
MMP-7 is the smallest metalloproteinase. Its unregulated activities and existence in serum are recently known to be tightly related with life-threatening disease such as cardiac disease and several cancers. The protein production is thought to be useful for its characterization and antibody generation. Although many attempts at bacterial expressions have been conducted, they were recovered as insoluble and inactive protein. In this study, after soluble expression, single-step purification and conversion to active protease, it was applied for the screening secretory metalloproteinase inhibitors in conditioned media of human cancer cells.