Systematic analysis of gene expression in human brains before and after death
Systematic analysis of gene expression in human brains before and after death
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DOI：
10.1186/gb-2005-6-13-r112
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发表时间：
2005-01-01
期刊：
影响因子：
12.3
通讯作者：
Khaitovich, Philipp
中科院分区：
文献类型：
--
作者：
Franz, Henriette;Ullmann, Claudia;Khaitovich, Philipp
背景：许多研究使用微阵列技术来研究与人类疾病、衰老和进化相关的基因表达的变化。绝大多数可用于研究的人体样本都是从已故的人身上获得的。这就提出了这样一个问题，即这些样本中的基因表达模式在多大程度上反映了活着的个体的基因表达模式。结果：在这里，我们比较了死后样本和手术干预期间收集的材料中人类大脑两个区域的基因表达模式。我们发现，死亡会导致超过10%的表达基因的表达发生显著变化。这些变化相对于它们的功能是非随机分布的。此外，我们在两个不同的大脑区域观察到了由于死亡而产生的相似的表达变化。因此，这两个大脑区域之间的基因表达差异模式在很大程度上不会受到死亡的影响，尽管在死后样本中差异的幅度减少了50%。此外，死亡诱导的变化对死后人脑样本之间的基因表达差异没有显著影响。结论：我们得出结论：死后人脑样本适合于研究人类的基因表达模式，但在解释单个基因的结果时应谨慎行事。
Background: Numerous studies have employed microarray techniques to study changes in gene expression in connection with human disease, aging and evolution. The vast majority of human samples available for research are obtained from deceased individuals. This raises questions about how well gene expression patterns in such samples reflect those of living individuals.Results: Here, we compare gene expression patterns in two human brain regions in postmortem samples and in material collected during surgical intervention. We find that death induces significant expression changes in more than 10% of all expressed genes. These changes are non-randomly distributed with respect to their function. Moreover, we observe similar expression changes due to death in two distinct brain regions. Consequently, the pattern of gene expression differences between the two brain regions is largely unaffected by death, although the magnitude of differences is reduced by 50% in postmortem samples. Furthermore, death-induced changes do not contribute significantly to gene expression variation among postmortem human brain samples.Conclusion: We conclude that postmortem human brain samples are suitable for investigating gene expression patterns in humans, but that caution is warranted in interpreting results for individual genes.