Integrative Proteomics and Metabolomics Analysis of Insect Larva Brain: Novel Insights into the Molecular Mechanism of Insect Wandering Behavior
Integrative Proteomics and Metabolomics Analysis of Insect Larva Brain: Novel Insights into the Molecular Mechanism of Insect Wandering Behavior
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DOI：
10.1021/acs.jproteome.5b00736
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发表时间：
2016-01-01
影响因子：
4.4
通讯作者：
Zhao, Ping
中科院分区：
文献类型：
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作者：
Li, Yi;Wang, Xin;Zhao, Ping
在变态之前，大多数全变态昆虫，比如这里研究的蚕，都经历了一个特殊的阶段，称为漫游阶段。这个阶段的昆虫通常表现出增强的运动活性（ELA）。ELA是至关重要的，因为它确保昆虫找到一个安全和合适的地方生活通过蛹阶段。游荡行为的生理机制尚不清楚。本研究采用蛋白质组学和代谢组学相结合的方法，对鳞翅目昆虫家蚕摄食期和游走期的脑组织进行了研究。使用LC MS/MS和GC MS，在所有我们确定了3004蛋白和37代谢产物在这两个阶段。其中465个蛋白质和22个代谢产物发生了变化。神经信号转导蛋白和代谢产物如神经递质、多巴胺能突触相关蛋白、谷氨酸等均发生明显改变，提示在游走期脑内发生了活跃的神经传导。我们还发现在流浪阶段多巴胺降解减少。主动神经传导的变化和多巴胺浓度的增加可能会诱发ELA。此外，参与泛素蛋白酶体系统和溶酶体途径的蛋白质被上调，揭示了大脑在变态过程中经历形态重塑。这些发现为昆虫游走行为的分子机制提供了新的见解。
Before metamorphosis, most holometabolous insects, such as the silkworm studied here, undergo a special phase called the wandering stage. Insects in this stage often display enhanced locomotor activity (ELA). ELA is vital because it ensures that the insect finds a safe and suitable place to live through the pupal stage. The physiological mechanisms of wandering behavior are still unclear. Here, we integrated proteomics and metabolomics approaches to analyze the brain of the lepidopteran insect, silkworm, at the feeding and wandering stages. Using LC MS/MS and GC MS, in all we identified 3004 proteins and 37 metabolites at these two stages. Among them, 465 proteins and 22 metabolites were changed. Neural signal transduction proteins and metabolites, such as neurofdament, dopaminergic synapse related proteins, and glutamic acid, were significantly altered, which suggested that active neural conduction occurred in the brain at the wandering stage. We also found decreased dopamine degradation at the wandering stage. The proposed changes in active neural conduction and increased dopamine concentration might induce ELA. In addition, proteins involved in the ubiquitin proteasome system and lysosome pathway were upregulated, revealing that the brain experiences morphological remodeling during metamorphosis. These findings yielded novel insights into the molecular mechanism underlying insect wandering behavior.