Annexin-I overexpression is associated with tumour progression and independently predicts inferior disease-specific and metastasis-free survival in urinary bladder urothelial carcinoma
Annexin-I overexpression is associated with tumour progression and independently predicts inferior disease-specific and metastasis-free survival in urinary bladder urothelial carcinoma
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DOI：
10.3109/00313020903434405
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发表时间：
2010-01-01
期刊：
影响因子：
4.5
通讯作者：
Wu, Ting-Feng
中科院分区：
文献类型：
--
作者：
Li, Chien-Feng;Shen, Kun-Hung;Wu, Ting-Feng
目标：在我们以前的研究中，比较蛋白质组学和免疫组织化学（IHC）表明，膜联蛋白-I（ANXA 1）是上调，在高级别膀胱尿路上皮癌（UBUC）相比，非高级别癌。然而，小样本量阻止了ANXA 1表达与肿瘤进展的进一步相关性。因此，在本研究中，81个主要的本地化UBUC标本的各种等级和原发性肿瘤（PT）的状态进行了检查ANXA 1的表达，以进一步确认蛋白质组学数据，并澄清ANXA 1的表达水平的UBUC.Methods预后的相关性：IHC实施调查ANXA 1蛋白表达在81个主要本地化UBUC标本。ANXA 1表达与肿瘤进展和预后的相关性进行了分析。结果：我们的数据表明，ANXA 1表达水平与pT状态升高（p < 0.001）和较高的组织学分级（p < 0.001）密切相关，表明ANXA 1可能与肿瘤进展有关。此外，在单变量水平，ANXA 1过表达，沿着较高的pT状态和组织学分级，显著预测疾病特异性生存期（DSS）和无转移生存期（MFS）。更重要的是，多因素分析显示，ANXA 1的过度表达和预后仍然显着DSS和MFS.Conclusion：上述结果加强了比较蛋白质组学的结果，并证实了ANXA 1在UBUC的预后作用。
Aims: In our previous studies, comparative proteomics and immunohistochemistry (IHC) demonstrated that annexin-I (ANXA1) is up-regulated in high grade urinary bladder urothelial carcinoma (UBUC) as compared to non-high grade carcinomas. However, the small sample size prohibited further correlation of ANXA1 expression to tumour progression. Therefore, in the present study, 81 primary localised UBUC specimens of various grades and primary tumour (pT) status were examined for ANXA1 expression to further confirm the proteomics data and to clarify the relevance of ANXA1 expression level to the prognosis of UBUC.Methods: IHC was implemented to investigate ANXA1 protein expression in 81 primary localised UBUC specimens. The association of ANXA1 expression with tumour progression and prognosis was analysed.Results: Our data demonstrated that the ANXA1 expression level was strongly associated with an escalated pT status (p < 0.001) and a higher histological grade (p < 0.001), suggesting that ANXA1 might be related to tumour progression. Moreover, at the univariate level, ANXA1 overexpression, along with higher pT status and histological grade, significantly predicted disease-specific survival (DSS) and metastasis-free survival (MFS). More importantly, multivariate analyses revealed that the association of ANXA1 overexpression and prognosis remained significant for both DSS and MFS.Conclusion: The above results reinforced the comparative proteomics results and confirmed the prognostic role of ANXA1 in UBUC.