MECHANISM OF THE ENZYMATIC-HYDROLYSIS OF CELLULOSE - EFFECTS OF MAJOR STRUCTURAL FEATURES OF CELLULOSE ON ENZYMATIC-HYDROLYSIS
MECHANISM OF THE ENZYMATIC-HYDROLYSIS OF CELLULOSE - EFFECTS OF MAJOR STRUCTURAL FEATURES OF CELLULOSE ON ENZYMATIC-HYDROLYSIS
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DOI：
10.1002/bit.260220113
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发表时间：
1980-01-01
影响因子：
3.8
通讯作者：
BEARDMORE, DH
中科院分区：
文献类型：
--
作者：
FAN, LT;LEE, YH;BEARDMORE, DH
纤维素对酶解的敏感性受纤维素材料的结构特性的影响。在这方面，纤维素纤维的结晶度和表面积可能是最重要的结构特征。本研究深入研究了这两种结构特征对[里氏木霉纤维素酶]酶解纤维素的相对影响以及纤维素在酶解过程中结构参数的变化。水解速率主要取决于纤维素的精细结构顺序，结晶度比表面积更能代表纤维素的精细结构顺序。对反应过程中结构参数变化的监测表明，表面面积并不是像之前所建议的那样，是减慢水解后期的主要限制因素。这些有关结构特征的信息用于阐明纤维素酶的作用方式。
The susceptibility of cellulose to enzymatic hydrolysis is affected by the structural features of cellulosic materials. The crystallinity and surface area of cellulose fibers are possibly the most important structural features in this regard. This study investigated in depth the relative effects of these 2 structural features upon the enzymatic hydrolysis of cellulose [by Trichoderma reesei cellulase] and the change of the structural parameters of cellulose during the course of hydrolysis. The hydrolysis rate was mainly dependent on the fine structural order of cellulose which can best be represented by the crystallinity rather than the simple surface area. Monitoring the changes in the structural parameters during the course of reaction showed that surface area is not a major limiting factor that slows hydrolysis in its late stages as was suggested. This information concerning structural features is used to elucidate the mode of action of cellulase.