ANTISENSE CCAAT ENHANCER-BINDING PROTEIN RNA SUPPRESSES COORDINATE GENE-EXPRESSION AND TRIGLYCERIDE ACCUMULATION DURING DIFFERENTIATION OF 3T3-L1 PREADIPOCYTES
ANTISENSE CCAAT ENHANCER-BINDING PROTEIN RNA SUPPRESSES COORDINATE GENE-EXPRESSION AND TRIGLYCERIDE ACCUMULATION DURING DIFFERENTIATION OF 3T3-L1 PREADIPOCYTES
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DOI：
10.1101/gad.6.4.533
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发表时间：
1992-04-01
影响因子：
10.5
通讯作者：
LANE, MD
中科院分区：
文献类型：
--
作者：
LIN, FT;LANE, MD
以往的研究表明，CCAAT/增强子结合蛋白（C/EBP）在3 T3-L1前脂肪细胞分化过程中协调脂肪细胞基因的表达。我们试图使用牛乳头状瘤病毒（BPV）载体选择性地阻断C/EBP的表达，以指导C/EBP cDNA的约0.4kb片段（反义方向）的转录，该片段含有编码蛋白质的碱性和亮氨酸拉链区的翻译序列5'。反义C/EBP RNA在3 T3-L1前脂肪细胞中的载体定向表达抑制了C/EBP mRNA和蛋白以及几种脂肪特异性mRNA的表达，并且还阻止了胞质甘油三酯的积累。通过用修饰的BPV载体转染表达反义RNA的细胞来完成“脂肪细胞表型”的拯救，所述修饰的BPV载体指导互补正义C/EBP RNA的转录。
Previous studies suggest that the CCAAT/enhancer-binding protein (C/EBP) functions in the coordinate expression of adipocyte genes during differentiation of 3T3-L1 preadipocytes. We sought to block expression of C/EBP selectively using a bovine papilloma virus (BPV) vector to direct transcription of a approximately 0.4-kb segment of C/EBP cDNA (in antisense orientation) containing translated sequence 5' to that encoding the basic and leucine zipper regions of the protein. Vector-directed expression of antisense C/EBP RNA in 3T3-L1 preadipocytes inhibited expression of C/EBP mRNA and protein, as well as several adipose-specific mRNAs, and also prevented cytoplasmic triglyceride accumulation. Rescue of the "adipocyte phenotype" was accomplished by transfection of cells expressing antisense RNA with a modified BPV vector that directs transcription of the complementary sense C/EBP RNA.