GENETIC AND BIOCHEMICAL-CHARACTERIZATION OF PERIPLASMIC-LEAKY MUTANTS OF ESCHERICHIA-COLI K-12
GENETIC AND BIOCHEMICAL-CHARACTERIZATION OF PERIPLASMIC-LEAKY MUTANTS OF ESCHERICHIA-COLI K-12
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DOI：
10.1128/jb.145.3.1351-1358.1981
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发表时间：
1981-01-01
影响因子：
3.2
通讯作者：
PORTALIER, RC
中科院分区：
文献类型：
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作者：
LAZZARONI, JC;PORTALIER, RC
经亚硝胺诱变后，分离到大肠杆菌K-12的周质渗漏突变株。他们将周质内酶释放到细胞外介质中。碱性磷酸酶在生长的早期指数阶段立即开始分泌，在稳定期可达到总酶产量的90%。漏水突变株对EDTA、胆酸及抗生素利福平、氯霉素、丝裂霉素C和氨苄西林敏感。它们对粘菌素E1也有抗性，对噬菌体途亚也有部分抗性。他们的基因特征表明，LKY突变位于Suc和Gal标记之间，靠近或位于tolPAB基因座上。对细胞膜成分的生化分析表明，周质泄漏突变体主要外膜蛋白OmpF的数量减少，而16,000道尔顿的外膜蛋白的数量增加。
Periplasmic-leaky mutants of E. coli K-12 were isolated after nitrosoguanidine-induced mutagenesis. They released periplasmic enzymes into the extracellular medium. Excretion of alkaline phosphatase, which started immediately in the early exponential phase of growth, could reach up to 90% of the total enzyme production in the stationary phase. Leaky mutants were sensitive to EDTA, cholic acid and the antibiotics rifampin, chloramphenicol, mitomycin C and ampicillin. They were also resistant to colicin E1 and partially resistant to phage TuIa. Their genetic characterization showed that the lky mutations mapped between the suc and gal markers, near or in the tolPAB locus. A biochemical analysis of cell envelope components showed that periplasmic-leaky mutants contained reduced amounts of major outer membrane protein OmpF and increased amounts of a 16,000-dalton outer membrane protein.