Effect of Low-level Vagus Nerve Stimulation on Cardiac Remodeling in a Rapid Atrial Pacing–induced Canine Model of Atrial Fibrillation
Effect of Low-level Vagus Nerve Stimulation on Cardiac Remodeling in a Rapid Atrial Pacing–induced Canine Model of Atrial Fibrillation
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DOI：
10.1097/fjc.0000000000000338
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发表时间：
2016-03
影响因子：
3
通讯作者：
Yanmei Lu;Juan Sun;Xianhui Zhou;Ling Zhang;Mei Ma;B. Tang
中科院分区：
文献类型：
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作者：
Yanmei Lu;Juan Sun;Xianhui Zhou;Ling Zhang;Mei Ma;B. Tang
摘要：本研究旨在建立快速心房起搏诱导的犬心房颤动模型，研究低水平迷走神经刺激(LLVNS)对心房颤动的影响及其机制。成年比格犬随机分为假手术组(Sham组)、快速左心耳起搏12h组(起搏组)和起搏+LLVNS 12h组(LLVNS组)。所有犬在不同时间点进行左、右心房有效不应期测试，然后处死动物，取心房和右前神经节丛组织标本进行显微镜检查。随着起搏次数的增加，起搏组的平均有效不应期明显缩短。起搏组与左室结组比较差异有统计学意义(P<；0.001)。3组大鼠心房心肌组织中高碘酸-席夫阳性面积染色密度差异有统计学意义。神经生长因子在假手术组、低迷走神经结组的表达均低于起搏组(神经生长因子分别为(36.35±6.18)×1000、(86.35±5.63)×1000、(40.50±7.24)×1000m2/mm~2，P<0.001；3组NRTN分别为(39.28±7.8)×1000、(80.24±6.56)×1000、(40.45±6.97)×1000m2/mm~2，P<0.001)。因此，LLVNS不仅能逆转快起搏引起的犬心房电重构，而且对自主神经的重构有结构性作用和刺激作用。
Abstract: The aim of this study was to establish a rapid atrial pacing–induced canine model of atrial fibrillation in studying the effects of low-level vagus nerve stimulation (LLVNS) on atrial fibrillation and the underlying mechanisms for those effects. Adult beagle dogs were randomly assigned to 3 groups: a sham operation group (sham group), a fast left atrial appendage 12-hour pacing group (pacing group), and a 12-hour pacing + LLVNS group (LLVNS group). All dogs underwent tests for their left and right atrial effective refractory period at various time points, after which they were killed, and samples of atrial and anterior right ganglionated plexi tissue were removed and microscopically examined. As pacing times increased, the mean effective refractory period in the pacing group became significantly shortened. The pacing group and the LLVNS group did show significant differences (P < 0.001). Three groups showed significant differences in their atrial myocardial periodic acid–Schiff–positive area staining densities. Anterior right ganglionated plexi expressions of nerve growth factor and neurturin (NRTN) in the sham group and the LLVNS group were lower than those in the pacing group (nerve growth factor in 3 groups were (36.35 ± 6.18) × 1000, (86.35 ± 5.63) × 1000, and (40.50 ± 7.24) × 1000 m2/mm2, P < 0.001; NRTN in 3 groups were (39.28 ± 7.80) × 1000, (80.24 ± 6.56) × 1000, (40.45 ± 6.97) × 1000 m2/mm2, P < 0.001). Therefore, LLVNS not only reverses the effect of fast pacing–induced atrial electrical remodeling in dogs but also exerts structural effects and stimulates remodeling of autonomic nerves.