ROLE OF THE MACROPHAGE IN MAREKS-DISEASE - INVITRO AND INVIVO STUDIES
ROLE OF THE MACROPHAGE IN MAREKS-DISEASE - INVITRO AND INVIVO STUDIES
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DOI：
10.1002/ijc.2910230510
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发表时间：
1979-01-01
影响因子：
6.4
通讯作者：
WILDER, M
中科院分区：
文献类型：
--
作者：
HAFFER, K;SEVOIAN, M;WILDER, M
本研究分别对马立克氏病（MD）易感和抗性的S系和K系来亨鸡的巨噬细胞进行了研究，以确定巨噬细胞在MD感染、肿瘤发生和遗传抗性动态中的作用。体外研究表明，两株巨噬细胞对马立克氏病病毒（MDV）JM株和JM-1肿瘤细胞的反应相似。观察到巨噬细胞吞噬JM病毒，但未观察到吞噬的病毒在巨噬细胞内复制或诱导细胞膜的抗原性变化。JM-1肿瘤细胞的清除是通过细胞溶解和吞噬机制。体内选择性抑制巨噬细胞功能的抗巨噬细胞血清或台盼蓝接种导致显着升高的病毒滴度和增加的肿瘤发生相比，感染，非抑制或非感染的对照组。显然，遗传易感性或抗性MD所表现出的S-和K-株鸡，分别是不受控制的巨噬细胞水平。巨噬细胞在MD感染中的作用似乎是特异性的监视。
Macrophages from S- and K-strain Leghorn chickens, susceptible and resistant to Marek''s disease (MD), respectively, were studied to determine the macrophage contribution to the dynamics of MD infection, tumorigenesis and genetic resistance to this disease. In vitro studies demonstrated that macrophages from both strains were similar in their responses toward JM strain of Marek''s disease virus (MDV) and JM-1 tumor cells. Macrophages were observed to phagocytize JM virus but the interiorized virus was not seen to replicate within the macrophage or induce antigenic changes of the cell membrane. Clearance of JM-1 tumor cells was by both cytolytic and phagocytic mechanisms. In vivo selective suppression of macrophage functions by antimacrophage serum or trypan blue inoculations resulted in significantly elevated viral titers and increased tumorigenesis as compared to infected, non-suppressed or non-infected control groups. Apparently, genetic susceptibility or resistance to MD as exhibited by S- and K-strain chickens, respectively, is not controlled at the macrophage level. The role of the macrophage in MD infection appears to be specifically surveillance.