The Genome of the CTG(Ser1) Yeast Scheffersomyces stipitis Is Plastic.
The Genome of the CTG(Ser1) Yeast Scheffersomyces stipitis Is Plastic.
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CTG（SER1）酵母Scheffersomyces症状的基因组是塑料。
DOI：
10.1128/mbio.01871-21
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发表时间：
2021-10-26
期刊：
影响因子：
6.4
通讯作者：
Buscaino A
中科院分区：
文献类型：
--
作者：
Vega-Estévez S;Armitage A;Bates HJ;Harrison RJ;Buscaino A
微生物需要适应环境变化，基因组可塑性可以通过增加遗传多样性来快速适应恶劣环境。在这里，我们研究了CTG（Ser1）酵母Schillosomiastipitis的基因组可塑性，Schillosomiastipitis是一种具有巨大潜力的第二代生物燃料生产的生物体。我们证明了S. stipitis具有内在可塑性基因组，不同S.树干炎分离株的基因组具有不同的染色体组织。实时进化实验表明，S。树干炎基因组可塑性是常见和快速的，因为在体外进化实验后检测到具有适应性益处的广泛基因组变化。混合MinION Nanopore和Illumina基因组测序将反转录转座子鉴定为基因组多样性的主要驱动因素。事实上，在不同的S. stipitis分离株和基因组的反转录转座子富集区是染色体重排的位点。我们的研究结果为CTG（Ser1）酵母进化枝的适应策略提供了重要的见解，并对第二代生物燃料的开发具有重要意义。这些数据强调了基因组可塑性是发展可持续S的重要因素。用于第二代生物燃料生产的stipitis平台。
Microorganisms need to adapt to environmental changes, and genome plasticity can lead to rapid adaptation to hostile environments by increasing genetic diversity. Here, we investigate genome plasticity in the CTG(Ser1) yeast Scheffersomyces stipitis, an organism with an enormous potential for second-generation biofuel production. We demonstrate that S. stipitis has an intrinsically plastic genome and that different S. stipitis isolates have genomes with distinct chromosome organizations. Real-time evolution experiments show that S. stipitis genome plasticity is common and rapid since extensive genomic changes with fitness benefits are detected following in vitro evolution experiments. Hybrid MinION Nanopore and Illumina genome sequencing identify retrotransposons as major drivers of genome diversity. Indeed, the number and position of retrotransposons are different in different S. stipitis isolates, and retrotransposon-rich regions of the genome are sites of chromosome rearrangements. Our findings provide important insights into the adaptation strategies of the CTG(Ser1) yeast clade and have critical implications in the development of second-generation biofuels. These data highlight that genome plasticity is an essential factor for developing sustainable S. stipitis platforms for second-generation biofuels production.