Quantitative intracellular magnetic nanoparticle uptake measured by live cell magnetophoresis
Quantitative intracellular magnetic nanoparticle uptake measured by live cell magnetophoresis
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DOI：
10.1096/fj.07-105544
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发表时间：
2008-12-01
期刊：
影响因子：
4.8
通讯作者：
Zborowski, Maciej
中科院分区：
文献类型：
--
作者：
Jing, Ying;Mal, Niladri;Zborowski, Maciej
超顺磁性氧化铁（SPIO）颗粒已成功地用作细胞内造影剂的核MRI细胞跟踪在体内。我们提出了一种利用细胞磁电泳检测活细胞内SPIO胶体摄取的方法，该方法在测量细胞内MRI对比剂摄取方面具有潜在的应用。通过测量细胞磁泳动率的平均值和分布的变化，以及与未操作的阴性对照相比时磁性阳性细胞群体频率的伴随变化，对该方法进行评价。使用基于树枝状聚合物、脂质和聚乙烯亚胺化合物的七种不同的转染剂（TA）-SPIO复合物作为测试标准，与3种不同的细胞类型组合：间充质干细胞、心脏成纤维细胞和培养的KG-1a造血干细胞。转染醇（TRA）-SPIO孵育导致最高频率的磁性阳性细胞（> 90%），而Fugene 6（FUG）-SPIO孵育最低，低于单独使用SPIO时的频率。一个高度规则的细胞磁泳过程是服从细胞内铁质量计算。在所有研究的细胞类型中，结果与其他报告一致。细胞磁泳取决于高自旋铁物质的存在，因此预期与细胞MRI对比度水平直接相关。
Superparamagnetic iron oxide (SPIO) particles have been used successfully as an intracellular contrast agent for nuclear MRI cell tracking in vivo. We present a method of detecting intracellular SPIO colloid uptake in live cells using cell magnetophoresis, with potential applications in measuring intracellular MRI contrast uptake. The method was evaluated by measuring shifts in mean and distribution of the cell magnetophoretic mobility, and the concomitant changes in population frequency of the magnetically positive cells when compared to the unmanipulated negative control. Seven different transfection agent (TA) -SPIO complexes based on dendrimer, lipid, and polyethylenimine compounds were used as test standards, in combination with 3 different cell types: mesenchymal stem cells, cardiac fibroblasts, and cultured KG-1a hematopoietic stem cells. Transfectol (TRA) -SPIO incubation resulted in the highest frequency of magnetically positive cells (> 90%), and Fugene 6 (FUG) -SPIO incubation the lowest, below that when using SPIO alone. A highly regular process of cell magnetophoresis was amenable to intracellular iron mass calculations. The results were consistent in all the cell types studied and with other reports. The cell magnetophoresis depends on the presence of high- spin iron species and is therefore expected to be directly related to the cell MRI contrast level.