Identification of the filtrable leukocyte‐transforming factor of qimr‐wil cells as herpes‐like virus
Identification of the filtrable leukocyte‐transforming factor of qimr‐wil cells as herpes‐like virus
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qimr-wil细胞的可过滤白细胞转化因子鉴定为疱疹样病毒
DOI：
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发表时间：
1969
影响因子：
6.4
通讯作者：
W. Scott
中科院分区：
文献类型：
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作者：
J. H. Pope;M. Horne;W. Scott
QIMR-WIL人白细胞系含有可转化胎儿人白细胞并允许建立细胞系的可过滤因子。该因子类似于疱疹病毒组的成员，其大小通过过滤确定，并在其对高温和乙醚的敏感性。该因子的转化活性被含有QIMR-WIL系疱疹样病毒抗体的人血清完全中和，但未被不含该抗体的对照人血清完全中和。得出结论，QIMR-WIL转化因子是疱疹样病毒。伯基特淋巴瘤细胞系QIMR-戈尔（其也携带疱疹样病毒）的传代也会产生类似的转化。与来自QIMR-WIL细胞的病毒（23天）相比，来自QIMR-戈尔细胞的病毒（49天）发生的白细胞增殖延迟，表明存在毒株差异。
The QIMR‐WIL human leukocyte cell line contains a filtrable factor which transforms foetal human leukocytes and allows establishment of cell lines. The factor resembled members of the Herpesvirus group in its size as determined by filtration and in its sensitivity to high temperature and ether. The transforming activity of the factor was completely neutralized by human sera containing antibody to the herpes‐like virus of the QIMR‐WIL line but not by control human sera without this antibody. It was concluded that the QIMR‐WIL transforming factor was the herpes‐like virus. Similar transformation was effected by filtrates of the Burkitt lymphoma line QIMR‐GOR which also carries herpes‐like virus. The delayed onset of leukocyte proliferation occurring with virus from QIMR‐GOR cells (49 days) compared with virus from QIMR‐WIL cells (23 days) suggests that strain differences exist.