Live imaging of heart tube development in mouse reveals alternating phases of cardiac differentiation and morphogenesis
Live imaging of heart tube development in mouse reveals alternating phases of cardiac differentiation and morphogenesis
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DOI：
10.7554/elife.30668
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发表时间：
2017-12-05
期刊：
影响因子：
7.7
通讯作者：
Torres, Miguel
中科院分区：
文献类型：
--
作者：
Ivanovitch, Kenzo;Temino, Susana;Torres, Miguel
在脊椎动物心脏发育过程中，两个祖细胞群体，第一和第二心脏场（FHF，SHF），依次有助于心管（HT）的纵向细分，其中FHF贡献左心室和部分心房，SHF贡献心脏的其余部分。在这里，我们通过跟踪小鼠胚胎活体分析中的单个细胞来研究心脏分化和形态发生的动力学。我们报告说，在初始阶段，FHF前体细胞迅速分化，形成心脏新月形，而有限的形态发生。在第二阶段，没有分化发生，而广泛的形态发生，包括内脏中胚层滑动内胚层，结果在HT形成。在第三阶段，心脏前体分化恢复并有助于SHF衍生区域和HT的背侧闭合。这些结果揭示了HT形成过程中形态发生和分化之间的组织水平协调，并为理解心脏发育提供了一个新的框架。
During vertebrate heart development, two progenitor populations, first and second heart fields (FHF, SHF), sequentially contribute to longitudinal subdivisions of the heart tube (HT), with the FHF contributing the left ventricle and part of the atria, and the SHF the rest of the heart. Here, we study the dynamics of cardiac differentiation and morphogenesis by tracking individual cells in live analysis of mouse embryos. We report that during an initial phase, FHF precursors differentiate rapidly to form a cardiac crescent, while limited morphogenesis takes place. In a second phase, no differentiation occurs while extensive morphogenesis, including splanchnic mesoderm sliding over the endoderm, results in HT formation. In a third phase, cardiac precursor differentiation resumes and contributes to SHF-derived regions and the dorsal closure of the HT. These results reveal tissue-level coordination between morphogenesis and differentiation during HT formation and provide a new framework to understand heart development.