Perturbations of gut microbiome genes in infants with atopic dermatitis according to feeding type
Perturbations of gut microbiome genes in infants with atopic dermatitis according to feeding type
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DOI：
10.1016/j.jaci.2017.11.045
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发表时间：
2018-04-01
影响因子：
14.2
通讯作者：
Hong, Soo-Jong
中科院分区：
文献类型：
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作者：
Lee, Min-Jung;Kang, Mi-Jin;Hong, Soo-Jong
背景资料：婴儿肠道微生物群的紊乱可以影响免疫系统的发育，并与过敏性疾病的风险有关。目的：我们试图了解肠道微生物群在特应性皮炎（AD）患者中的作用。方法：采用焦磷酸测序法对129例6个月龄婴儿（包括66例健康婴儿和63例AD婴儿）的粪便样本进行肠道菌群组成分析。通过全宏基因组测序分析肠道微生物组的功能概况（20名对照受试者和20名AD患者）。结果：不同喂养方式的6月龄婴儿肠道菌群存在差异，母乳喂养和混合喂养婴儿肠道菌群均以双歧杆菌属和埃希氏菌/韦荣球菌属为主。AD患儿粪便中的细菌细胞数量低于对照组。虽然在16 S rRNA基因结果中没有特定的分类群与AD直接相关，但全宏基因组分析揭示了与免疫发育相关的功能基因的差异。由于粘蛋白降解细菌的定植减少，氧化磷酸化、磷脂酰肌醇3-激酶-Akt信号传导、雌激素信号传导、核苷酸结合结构域样受体信号传导以及抗原加工和呈递的基因减少（嗜粘蛋白阿克曼氏菌，活泼瘤胃球菌，和毛螺菌科细菌2_1_58FAA）与AD组的免疫发育迟缓显著相关结论：肠道微生物组的改变可能与AD相关，因为不同的细菌基因可以调节宿主免疫细胞功能。
Background: Perturbations of the infant gut microbiota can shape development of the immune system and link to the risk of allergic diseases.Objective: We sought to understand the role of the gutmicrobiome in patients with atopic dermatitis (AD). The metagenome of the infant gut microbiome was analyzed according to feeding types.Methods: Composition of the gut microbiota was analyzed in fecal samples from 129 infants (6 months old) by using pyrosequencing, including 66 healthy infants and 63 infants with AD. The functional profile of the gut microbiome was analyzed by means of whole-metagenome sequencing (20 control subjects and 20 patients with AD). In addition, the total number of bacteria in the feces was determined by using real-time PCR.Results: The gut microbiome of 6-month-old infants was different based on feeding types, and 2 microbiota groups (Bifidobacterium species-dominated and Escherichia/Veillonella species-dominated groups) were found in breast-fed and mixed-fed infants. Bacterial cell amounts in the feces were lower in infants with AD than in control infants. Although no specific taxa directly correlated with AD in 16S rRNA gene results, whole-metagenome analysis revealed differences in functional genes related to immune development. The reduction in genes for oxidative phosphorylation, phosphatidylinositol 3-kinase-Akt signaling, estrogen signaling, nucleotide-binding domain-like receptor signaling, and antigen processing and presentation induced by reduced colonization of mucin-degrading bacteria (Akkermansia muciniphila, Ruminococcus gnavus, and Lachnospiraceae bacterium 2_1_58FAA) was significantly associated with stunted immune development in the AD group compared with the control group (P < .05).Conclusions: Alterations in the gut microbiome can be associated with AD because of different bacterial genes that can modulate host immune cell function.