COLD-INDUCED GLYCEROL ACCUMULATION BY OSTRINIA-NUBILALIS LARVAE IS DEVELOPMENTALLY REGULATED

COLD-INDUCED GLYCEROL ACCUMULATION BY OSTRINIA-NUBILALIS LARVAE IS DEVELOPMENTALLY REGULATED
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DOI:
10.1016/0022-1910(84)90084-2
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发表时间:
1984-01-01
影响因子:
2.2
通讯作者:
YIN, CM
YIN, CM
中科院分区:
农林科学3区
文献类型:
--
作者:
NORDIN, JH;ZHENG, C;YIN, CM

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O.将在非滞育和滞育诱导条件下饲养的nubilalis幼虫在5 ℃下冷藏。C的不同时期和他们的血淋巴甘油浓度测定酶。第5(终)龄积累甘油的能力是依赖于冷胁迫,但不是滞育状态。这种反应是独立的任何冷诱导释放的头部或胸部激素。O.发现nubilalis幼虫表达冷诱导的甘油积累需要从4 - 5龄蜕皮。卵和2、3、4龄幼虫完全不能发育。这些结果表明,在生化水平上,O. nubilalis来证明这种对冷应激的反应。
O. nubilalis larvae reared under both nondiapause and diapause-inducing conditions were chilled at 5.degree. C for various periods and their hemolymph glycerol concentrations were measured enzymatically. The ability of 5th (final) instars to accumulate glycerol was dependent upon cold stress but not the diapause state. This response was independent of any cold-induced release of cephalic or thoracic hormones. The capacity of O. nubilalis larvae to express cold-induced glycerol accumulation was found to require ecdysis from the 4th-5th instar. Eggs as well as 2nd, 3rd and 4th instars were completely incompetent. These results indicate that, at the biochemical level, a specific developmental program or sequence is required for O. nubilalis to demonstrate this response to cold stress.