THE 64-KDA PROTEIN THAT ASSOCIATES WITH THE PLATELET-DERIVED GROWTH-FACTOR RECEPTOR BETA-SUBUNIT VIA TYR-1009 IS THE SH2-CONTAINING PHOSPHOTYROSINE PHOSPHATASE SYP
THE 64-KDA PROTEIN THAT ASSOCIATES WITH THE PLATELET-DERIVED GROWTH-FACTOR RECEPTOR BETA-SUBUNIT VIA TYR-1009 IS THE SH2-CONTAINING PHOSPHOTYROSINE PHOSPHATASE SYP
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DOI：
10.1073/pnas.90.15.6939
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发表时间：
1993-08-01
影响因子：
11.1
通讯作者：
VALIUS, M
中科院分区：
文献类型：
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作者：
KAZLAUSKAS, A;FENG, GS;VALIUS, M
配体刺激的血小板源性生长因子受体 (PDGFR) β 亚基的自磷酸化为含有 SH2 的蛋白质创建了许多结合位点。 PDGFR 相关蛋白之一是身份和功能未知的 64 kDa 蛋白。我们提供的数据表明，与激活的 PDGFR 相关的 64-kDa 蛋白是 Syp（也称为 SH-PTP2、PTP-1D 或 SH-PTP3），它是普遍表达的包含 64-kDa SH2 的蛋白酪氨酸磷酸酶。 PDGFR C 末端的 Tyr-1009 磷酸化是 Syp 稳定结合所必需的，表明该残基的磷酸化为 Syp SH2 结构域创建了一个结合位点。尽管 Syp 与 PDGFR 稳定结合，但 PDGF 刺激的 Syp 酪氨酸磷酸化不需要此事件。这些数据提出了一个有趣的可能性，即蛋白酪氨酸磷酸酶有助于源自受体酪氨酸激酶（例如 PDGFR）的生物信号的细胞内中继。
Ligand-stimulated autophosphorylation of the platelet-derived growth factor receptor (PDGFR) beta subunit creates a number of binding sites for SH2-containing proteins. One of the PDGFR-associated proteins is a 64-kDa protein of unknown identity and function. We present data indicating that the 64-kDa protein that associates with the activated PDGFR is Syp (also called SH-PTP2, PTP-1D, or SH-PTP3), the ubiquitously expressed 64-kDa SH2-containing protein-tyrosine phosphatase. Phosphorylation of Tyr-1009 in the C terminus of the PDGFR is required for the stable association of Syp, suggesting that phosphorylation of this residue creates a binding site for the Syp SH2 domains. Although Syp stably associates with the PDGFR, this event is not required for PDGF-stimulated tyrosine phosphorylation of Syp. These data raise the interesting possibility that protein-tyrosine phosphatases contribute to the intracellular relay of biological signals originating from receptor tyrosine kinases such as the PDGFR.