Wheat and barley allergens associated with baker's asthma. Glycosylated subunits of the alpha-amylase-inhibitor family have enhanced IgE-binding capacity.
Wheat and barley allergens associated with baker's asthma. Glycosylated subunits of the alpha-amylase-inhibitor family have enhanced IgE-binding capacity.
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小麦和大麦过敏原与面包师哮喘有关。
DOI：
10.1042/bj2810401
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
G. Salcedo
中科院分区：
文献类型：
--
作者：
R. Sánchez;L. Gómez;D. Barber;C. López;A. Armentia;G. Salcedo
一种名为CM16*的16 kDa蛋白已被鉴定为先前报道的WTAI-CM16的糖基化形式，CM16是小麦四聚体α -淀粉酶抑制剂的一个亚基。BTAI-CMb的糖基化形式（CMb*），来自大麦的等效抑制剂亚基，也被发现具有显著增强的ige结合能力。总的来说，14个纯化的α -淀粉酶/胰蛋白酶抑制剂家族成员在点印迹试验中显示出非常不同的ige结合能力。发现糖基化成分CM16*、CMb*和先前描述的大麦非糖基化14.5 kDa过敏原（更名为BMAI-1）是最强的过敏原。
A 16 kDa protein, designated CM16*, which strongly binds IgE from baker's-asthma patients has been identified as a glycosylated form of the previously reported WTAI-CM16, which is a subunit of the wheat tetrameric alpha-amylase inhibitor. A glycosylated form (CMb*) of BTAI-CMb, the equivalent inhibitor subunit from barley, has been also found to have significantly enhanced IgE-binding capacity. In all, 14 purified members of the alpha-amylase/trypsin-inhibitor family showed very different IgE-binding capacities when tested by a dot-blot assay. The glycosylated components CM16*, CMb* and the previously described non-glycosylated 14.5 kDa allergen from barley (renamed BMAI-1) were found to be the strongest allergens.