Lack of detectable genetic recombination on the X chromosome during the parthenogenetic production of female and male aphids
Lack of detectable genetic recombination on the X chromosome during the parthenogenetic production of female and male aphids
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DOI：
10.1017/s0016672302005657
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发表时间：
2002-06-01
影响因子：
1.5
通讯作者：
Sunnucks, P
中科院分区：
文献类型：
--
作者：
Hales, DF;Wilson, ACC;Sunnucks, P
利用多态微卫星标记，研究了大siphini族蚜虫X染色体在孤雌生殖过程中的重组。我们研究了X染色体，因为它占基因组的25%，之前的细胞学观察表明染色体配对和核仁组织者（NOR）异型性表明重组，尽管常染色体并非如此。在3个信息性x连锁位点对564个具有三种不同生殖模式（周期性孤雌生殖、专性孤雌生殖和专性孤雌伴雄生殖）的Myzus无性系进行了基因分型。此外，从包含所有雄性生殖策略的克隆中孤雌生殖产生的雄性被基因分型。其中391只桃蚜雄性在3个x连锁位点上被分型，538只来自Sitobion克隆的雄性在5个信息丰富的x连锁位点上被分型。我们的研究结果表明，在Macrosiphini部落的单性生殖世代中，没有偏离克隆性：在单性生殖产生的雄性或雌性中没有观察到重组基因型。
We used polymorphic microsatellite markers to look for recombination during parthenogenetic oogenesis between the X chromosomes of aphids of the tribe Macrosiphini. We examined the X chromosome because it comprises similar to25% of the genome and previous cytological observations of chromosome pairing and nucleolar organizer (NOR) heteromorphism suggest recombination, although the same is not true for autosomes. A total of 564 parthenogenetic females of Myzus clones with three distinct reproductive modes (cyclical parthenogenesis, obligate parthenogenesis and obligate parthenogenesis with male production) were genotyped at three informative X-linked loci. Also, parthenogenetically produced males from clones encompassing the full range of male-producing reproductive strategies were genotyped. These included 391 Myzus persicae males that were genotyped at three X-linked loci and 538 males from Sitobion clones that were genotyped at five informative X-linked loci. Our results show no departure from clonality in parthenogenetic generations of aphids of the tribe Macrosiphini: no recombinant genotypes were observed in parthenogenetically produced males or females.