Consistent effects of nitrogen fertilization on soil bacterial communities in black soils for two crop seasons in China.
Consistent effects of nitrogen fertilization on soil bacterial communities in black soils for two crop seasons in China.
复制标题
施氮肥对中国黑土两季土壤细菌群落的一致性影响
DOI：
10.1038/s41598-017-03539-6
复制
发表时间：
2017-06-12
影响因子：
4.6
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Zhou J;Jiang X;Wei D;Zhao B;Ma M;Chen S;Cao F;Shen D;Guan D;Li J
长期施用无机氮肥对东北黑土细菌群落结构有较大影响。目前还不清楚氮如何影响细菌群落在连续两个作物季节在同一领域的这种土壤类型。以哈尔滨市长期定位施肥试验田的土壤为研究对象，设置了3个氮素梯度。我们应用测序和定量PCR靶向16S rRNA基因，以检查细菌群落的变化，并测试一致的变化和驱动因素细菌对高氮添加的反应。施氮降低了土壤pH值和细菌16SrDNA拷贝数，增加了土壤氮素含量和作物产量。氮添加一贯降低细菌多样性和改变细菌群落组成，通过增加变形菌的相对丰度，并减少在两个季节的酸杆菌和硝化螺菌。在丰富的类和属的一致的变化，以及跨两个季节的细菌群落的结构进行了观察。研究结果表明，连续2年的氮素投入对土壤细菌群落的丰富度、多样性和组成有一致的影响，氮素投入和随后的土壤变化是影响土壤细菌群落结构的重要因素。
Long-term use of inorganic nitrogen (N) fertilization has greatly influenced the bacterial community in black soil of northeast China. It is unclear how N affects the bacterial community in two successive crop seasons in the same field for this soil type. We sampled soils from a long-term fertilizer experimental field in Harbin city with three N gradients. We applied sequencing and quantitative PCR targeting at the 16S rRNA gene to examine shifts in bacterial communities and test consistent shifts and driving-factors bacterial responses to elevated N additions. N addition decreased soil pH and bacterial 16S rDNA copy numbers, and increased soil N and crop yield. N addition consistently decreased bacterial diversity and altered bacterial community composition, by increasing the relative abundance of Proteobacteria, and decreasing that of Acidobacteria and Nitrospirae in both seasons. Consistent changes in the abundant classes and genera, and the structure of the bacterial communities across both seasons were observed. Our results suggest that increases in N inputs had consistent effects on the richness, diversity and composition of soil bacterial communities across the crop seasons in two continuous years, and the N addition and the subsequent edaphic changes were important factors in shaping bacterial community structures.