Adaptation of microscopy with ultraviolet surface excitation for enhancing STEM and undergraduate education
Adaptation of microscopy with ultraviolet surface excitation for enhancing STEM and undergraduate education
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DOI：
10.1117/1.jbo.23.12.121603
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发表时间：
2018-12-01
影响因子：
3.5
通讯作者：
Demos, Stavros G.
中科院分区：
文献类型：
--
作者：
Huang, Chi Z. R.;Wood, Ronald W.;Demos, Stavros G.
显微镜与紫外线表面激发（MUSE）的研究作为一种手段，以提高课程和教育的基础上，简单的使用，廉价的硬件的结合，和最简单的方法的组织准备在生命科学。紫外线激发实际上取代了组织切片，因为它在单细胞水平上只穿透组织表面以下几微米，防止产生散焦光。虽然可以使用组织自体荧光，但可以通过短暂浸入选择性地突出不同细胞区室的无毒荧光染料溶液中来增强图像质量和内容。安全的混合染料粉末组合已被开发出来，为学生谁拥有最低的实验室专业知识与快速组织制备一步组织染色过程。(C)作者。由SPIE在知识共享署名3.0未移植许可下发布。
Microscopy with ultraviolet surface excitation (MUSE) is investigated as a means to enhance curricula and education in the life sciences based on simplicity of use, the incorporation of inexpensive hardware, and the simplest methods of tissue preparation. Ultraviolet excitation in effect replaces tissue sectioning because it penetrates only a few micrometers below the tissue surface at the single cell level, preventing the generation of out-of-focus light. Although tissue autofluorescence may be used, image quality and content can be enhanced by a brief immersion in a solution of nontoxic fluorescent dyes that selectively highlight different cellular compartments. Safe mixed-dye powder combinations have been developed to provide students who have minimal lab proficiencies with a one-step tissue staining process for rapid tissue preparation. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.