Biofilm detachment by self-collapsing air microbubbles: a potential chemical-free cleaning technology for membrane biofouling
Biofilm detachment by self-collapsing air microbubbles: a potential chemical-free cleaning technology for membrane biofouling
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DOI：
10.1039/c1jm14439a
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Liu, Yu
中科院分区：
文献类型：
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作者：
Agarwal, Ashutosh;Xu, Huijuan;Liu, Yu
微气泡（MB）已经被认为是能够通过收缩和随后的自塌陷现象产生压力波的。在本研究中，我们已经调查了潜在的空气MBs的生物膜分离从尼龙膜表面相比，化学清洗次氯酸钠（NaOCl）。空气微泡1h后，观察到约88%的固定生物质脱落，而在没有微泡的对照实验中，仅实现了10%的生物膜脱落。用共聚焦激光扫描显微镜（CLSM）拍摄的图像清楚地显示，生物膜中几乎所有的胞外多糖和蛋白质都从膜表面去除，表明生物膜的胞外聚合物基质完全破坏。进一步证明了微泡在去除固定的生物质和胞外多糖和蛋白质方面比用0.5%NaOCl溶液的化学清洗有效得多。本研究提供的实验证据表明，自塌陷空气MBs是一种无化学和生态友好的生物膜分离技术。
Microbubbles (MBs) have been known for their ability to generate pressure waves through shrinking and subsequent self-collapsing phenomenon. In the present study, we have investigated the potential of air MBs for biofilm detachment from a nylon membrane surface in comparison to chemical cleaning by sodium hypochloride (NaOCl). About 88% of fixed biomass detachment was observed after 1 h air microbubbling, while only 10% of biofilm detachment was achieved in the control experiment without microbubbles. Images taken with a confocal laser scanning microscope (CLSM) clearly showed that nearly all extracellular polysaccharides and proteins in biofilms were removed from the membrane surface, indicating a complete disruption of the extracellular polymeric matrix of biofilms. It was further demonstrated that microbubbling is much more efficient than chemical cleaning with 0.5% NaOCl solution in terms of removal of fixed biomass and extracellular polysaccharides and proteins. This study provides experimental evidence showing that self-collapsing air MBs is a chemical-free and eco-friendly technology for biofilm detachment.