IMPROVED CYCLING ASSAY FOR NICOTINAMIDE ADENINE-DINUCLEOTIDE
IMPROVED CYCLING ASSAY FOR NICOTINAMIDE ADENINE-DINUCLEOTIDE
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DOI：
10.1016/0003-2697(73)90094-8
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发表时间：
1973-01-01
影响因子：
2.9
通讯作者：
SWAN, M
中科院分区：
文献类型：
--
作者：
BERNOFSKY, C;SWAN, M
已发现使用噻唑蓝作为末端电子受体的NAD的新循环测定法比使用2，6-二氯酚靛酚的更成熟的程序具有显着优势。与噻唑蓝，循环试验是线性的NAD在皮摩尔水平，并与时间至少为120分钟。相反，与2，6-二氯酚靛酚作为终端受体，循环试验偏离显着的线性在皮摩尔水平的NAD，和反应速率成为线性较短的时间内，作为NAD的水平增加。数据提供了一个基础，选择最佳的测定条件，使用新的噻唑蓝循环技术。
A new cycling assay for NAD that uses thiazolyl blue as a terminal electron acceptor has been found to offer significant advantages over the more established procedure that employs 2,6-dichlorophenolindophenol. With thiazolyl blue, the cycling assay is linear with NAD at picomole levels, and with time for at least 120 min. In contrast, with 2,6-dichlorophenolindophenol as terminal acceptor, the cycling assay deviates considerably from linearity at picomole levels of NAD, and the reaction rates become linear for shorter periods of time as the level of NAD increases. Data are given which provide a basis for choosing optimal assay conditions using the new thiazolyl blue cycling technique.