Allergen-induced recruitment of Fc epsilon RI+ eosinophils in human atopic skin
Allergen-induced recruitment of Fc epsilon RI+ eosinophils in human atopic skin
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DOI：
10.1002/eji.1830270527
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发表时间：
1997-03-01
影响因子：
5.4
通讯作者：
Kay, AB
中科院分区：
文献类型：
--
作者：
Barata, LT;Ying, S;Kay, AB
我们尝试在注射过敏原或稀释剂对照后6、24和48 h活检的特应性受试者中鉴定皮肤晚期反应中的Fc ε RI+嗜酸性粒细胞。与稀释剂部位相比，变应原注射部位的嗜酸性粒细胞数量显著增加，在6 - 24 h之间达到峰值，其中约20- 30%表达Fc ε RI α、β和γ链的mRNA，如原位杂交所示。使用单克隆或多克隆抗ct链抗体，在研究的所有时间点，Fc β RI α蛋白也共定位于约50-80%的嗜酸性粒细胞。我们还观察到Fc ε RI+（997（+））/EG 2（+）嗜酸性粒细胞的数量与晚期反应的程度之间存在显著相关性（r = 0.89; p = 0.02）。因此，在特应性受试者中，有相当比例的嗜酸性粒细胞浸润在变应原诱导的过敏组织反应部位，表达Fc ε RI。结果表明，高亲和力IgE受体可能在体内嗜酸性粒细胞分泌过程中发挥作用。
We have attempted to identify Fc epsilon RI+ eosinophils in cutaneous late-phase reaction in atopic subjects biopsied at 6, 24 and 48 h after the injection of either allergen or a diluent control. Compared to the diluent sites, allergen-injected sites had significantly increased numbers of eosinophils, peaking between 6 and 24 h, of which approximately 20-30 % expressed mRNA for the alpha, beta, and gamma chains of Fc epsilon RI as shown by in situ hybridization. Using either a monoclonal or a polyclonal anti-ct chain antibody, the Fc epsilon RI alpha protein also co-localized to approximately 50-80% of eosinophils at all time points studied. We also observed a significant correlation (r = 0.89; p = 0.02) between the numbers of Fc epsilon RI+ (997(+))/EG2(+) eosinophils and the magnitude of the late-phase reaction. Thus, a significant proportion of eosinophils infiltrating the site of allergen-induced allergic tissue reactions in atopic subjects express Fc epsilon RI. The findings show that high-affinity IgE receptors may play a role in eosinophil secretory processes in vivo.