Genetic characterization of viable Toxoplasma gondii isolates from stray dogs from Giza, Egypt
Genetic characterization of viable Toxoplasma gondii isolates from stray dogs from Giza, Egypt
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DOI：
10.1016/j.vetpar.2012.12.007
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发表时间：
2013-03-31
影响因子：
2.6
通讯作者：
Dubey, J. P.
中科院分区：
文献类型：
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作者：
El Behairy, A. M.;Choudhary, S.;Dubey, J. P.
流浪狗被认为是弓形虫流行病学的哨兵，因为它们是肉食动物，吃各种食物，包括垃圾。在本研究中，对来自埃及吉萨的 51 只流浪狗（Coolsamilis）的组织和血清进行了弓形虫感染检查。通过改良凝集试验（MAT）检查血清中弓形虫抗体； 51 例中有 50 例（98%）呈血清反应阳性，滴度为 4 例 20、4 例 40、1 例 80、8 例 100、17 例 200、11 例 400、5 例 800 或更高。在小鼠体内对 43 只血清反应呈阳性的狗的心脏进行了生物测定。从 22 只狗身上分离出活的弓形虫；这些分离株被命名为 TgDogEg1 至 TgDogEg22。使用 10 个 PCR 限制性片段长度多态性标记（SAG1、SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、1358、PK1 和 Apico）对从 22 个分离株的细胞培养物衍生的速殖子中分离出的 DNA 进行基因分型。结果显示三种基因型和一种混合感染。三种基因型是 ToxoDB PCR-RFLP #2（III 型，4 个分离株）、#3（II 型变体，11 个分离株）、#20（6 个分离株）、1 个混合感染。这些结果揭示了来自埃及吉萨的流浪狗中弓形虫克隆型 II、III 和 ToxoDB #20 谱系的优势。由 Elsevier B.V. 出版
Stray dogs are considered as sentinels in the epidemiology of Toxoplasma gondii because they are carnivores and eat variety of foods, including garbage. In the present study, tissues and sera of 51 stray dogs (Cools familiaris) from Giza, Egypt were examined for T. gondii infection. Sera were examined for antibodies to T. gondii by the modified agglutination test (MAT); 50 of 51 (98%) were seropositive with titers of 20 in four, 40 in four, 80 in one, 100 in eight, 200 in 17, 400 in 11, 800 or higher in five. Hearts of 43 seropositive dogs were bioassayed in mice. Viable T. gondii was isolated from 22 dogs; these isolates were designated TgDogEg1 to TgDogEg22. DNA isolated from cell culture derived tachyzoites of 22 isolates was genotyped using 10 PCR-restriction fragment length polymorphism markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, 1358, PK1, and Apico). The results revealed three genotypes and one mixed infection. The three genotypes are ToxoDB PCR-RFLP #2 (type III, four isolates), #3 (type II variant, 11 isolates), #20 (six isolates), 1 mixed infection. These results revealed the dominance of clonal type II, III and ToxoDB #20 lineages of T. gondii in stray dogs from Giza, Egypt. Published by Elsevier B.V.