Automation of specimen selection and data acquisition for protein electron crystallography
Automation of specimen selection and data acquisition for protein electron crystallography
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DOI：
10.1016/s0304-3991(98)00022-9
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发表时间：
1998-07-01
期刊：
影响因子：
2.2
通讯作者：
Brisson, A
中科院分区：
文献类型：
--
作者：
Oostergetel, GT;Keegstra, W;Brisson, A
介绍了一种用于蛋白质电子晶体学的半自动样品选择和数据采集系统，该系统基于慢扫描CCD摄像机与透射电子显微镜相连，并由外部计算机控制。样品上的感兴趣区域以低放大率定位，随后在CCD相机上成像，使用与曝光模式中使用的剂量相比较小的剂量。从计算的图像傅立叶变换中衍射峰的出现来评价该区域的结晶质量。如果质量被认为是好的，图像可以记录在不同的模式，无论是在电影和使用CCD相机。使用该系统，可以获得显着的增益，定量和定性，在获取数据的电子晶体学的束敏感材料。(C)1998 Elsevier Science B.V.保留所有权利。
A system is presented for semi-automatic specimen selection and data acquisition for protein electron crystallography, based on a slow-scan CCD camera connected to a transmission electron microscope and control from an external computer. Areas of interest on the specimen are localised at low magnification and subsequently imaged on the CCD camera, using a dose which is small compared to the dose used in the exposure mode. The crystalline quality of the area is evaluated from the appearance of diffraction peaks in the calculated image Fourier transform. If the quality is considered good, images can then be recorded in different modes, both on film and using the CCD camera. Using this system a significant gain, both quantitatively and qualitatively, can be obtained in acquiring data for electron crystallography of beam-sensitive materials. (C) 1998 Elsevier Science B.V. All rights reserved.