CELL-DIVISION AND DEATH IN THE MOUSE BLASTOCYST BEFORE IMPLANTATION
CELL-DIVISION AND DEATH IN THE MOUSE BLASTOCYST BEFORE IMPLANTATION
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DOI：
10.1007/bf00375893
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发表时间：
1986-01-01
期刊：
影响因子：
--
通讯作者：
HUNTER, S
中科院分区：
文献类型：
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作者：
HANDYSIDE, AH;HUNTER, S
用两种多核苷酸特异性荧光染料分别标记小鼠囊胚滋养外胚层（TE）和内细胞团（ICM）细胞核，计数其细胞数。分析了从第4天早期形成到第5天植入初始阶段之间的间隔从子宫中回收的囊胚。TE细胞数量的增加最初是快速的，表明第六次分裂的一些同步，但逐渐放缓，并在第5天达到平台，可能是由于原发性巨细胞形成的开始。ICM细胞数量的增加比相应的TE细胞慢。结果，TE细胞数量增加了四倍多，而ICM细胞数量在此期间仅增加了一倍。虽然两个群体的细胞的有丝分裂指数稳步下降，但它们之间没有显著差异。因此，ICM细胞比例的下降可能是由于细胞死亡，首先在早期囊胚中检测到，主要位于ICM中。然而，此外，不能排除ICM细胞对上覆极性TE的贡献。
The numbers of cells in the trophectoderm (TE) and inner cell mass (ICM) of mouse blastocysts were counted by differentially labelling their nuclei with two polynucleotide-specific fluorochromes. Blastocysts recovered from the uterus at intervals between their formation early on Day 4 to the initial stages of implantation on day 5 were analysed. TE cell number increase was initially rapid, indicating some synchronisation of the sixth division, but slowed down progressively and plateaued on Day 5, possibly due to the onset of primary giant cell formation. ICM cell number increase was slower than the corresponding TE cells. As a result, TE cell number more than quadrupled, whereas ICM cell number only doubled over this period. Although the mitotic index of both populations of cells fell steadily, there was no significant difference between them. The decline in the proportion of ICM cells, therefore, is likely to be due to cell death, first detected in early blastocysts and predominantly located in the ICM. In addition, however, a contribution of ICM cells to the overlying polar TE cannot be excluded.