SSR mapping and confirmation of the QTL from PI96354 conditioning soybean resistance to southern root-knot nematode
SSR mapping and confirmation of the QTL from PI96354 conditioning soybean resistance to southern root-knot nematode
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DOI：
10.1007/s001220100672
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发表时间：
2001-12-01
影响因子：
5.4
通讯作者：
Boerma, HR
中科院分区：
文献类型：
--
作者：
Li, Z;Jakkula, L;Boerma, HR
根结线虫（Meloidogyne spp.）可导致美国南部产区大豆 [Glycine max (L.) Merr.] 严重减产。种植抗根结线虫品种是防止产量损失的最有效方法。 DNA标记辅助育种可能会加速根结线虫抗性品种的开发。 RFLP 标记先前已被用于鉴定通过抗性 PI96354 和易感性 'Bossier' 杂交产生的 F-2:3 大豆群体中赋予对南方根结线虫 [Meloidogyne_incognita (Kofoid 和 White) Chitwood] (Mi) 抗性的数量性状位点 (QTL)。据报道，连锁群（LG）O上的主要QTL调节了Mi瘿数变异的31%，而LG-G上的次要QTL调节了瘿数变异的14%。随着用于大豆改良的 SSR 标记的开发，更高水平的作图分辨率和半自动检测已成为可能。本研究的目的是：(1) 使用 SSR 标记增加 LG-O 和 LG-G 上 Mi 抗性 QTL 基因组区域的标记密度，以及 (2) 确认 QTL 在第二个群体和不同遗传背景 Ground 中的效果。使用SSR标记，LG-O上的QTL侧翼为Satt492和Satt358，LG-G上的QTL侧翼为Satt012和Satt505。利用两个 QTL 侧翼的 SSR 标记，在 PI96354X Bossier 的第二个 F-2:3 群体中进行标记辅助选择。结果证实了标记辅助选择预测 Mi 表型的有效性。通过筛选Prichard (3)XG93-9009的BC2F2群体，我们证实选择带有侧翼SSR标记的LG-G上的次要QTL将增强含有主要QTL（最有可能是Rmil）的品系的抗性。
Root-knot nematodes (Meloidogyne spp.) can cause severe yield loss of soybean [Glycine max (L.) Merr.] in the southern production region of the USA. Planting root-knot nematode-resistant cultivars is the most effective method of preventing yield loss. DNA marker-assisted breeding may accelerate the development of root-knot nematode-resistant cultivars. RFLP markers have previously been used to identify quantitative trait loci (QTLs) conferring resistance to southern root-knot nematode [Meloidogyne_incognita (Kofoid and White) Chitwood] (Mi) in a F-2:3 soybean population created by crossing the resistant PI96354 and the susceptible 'Bossier.' A major QTL On linkage group (LG) O conditioning 31% of the variation in Mi gall number and a minor QTL on LG-G conditioning 14% of the gall variation were reported. With the development of SSR markers for soybean improvement, a higher level of mapping resolution and semi-automated detection has become possible. The objectives of this research were: (1) to increase the marker density in the genomic regions of the QTLs for Mi resistance on LG-O and LG-G with SSR markers, and (2) to confirm the effect of the QTLs in a second population and a different genetic back, Ground. With SSR markers, the QTL on LG-O was flanked by Satt492 and Satt358, and on LG-G by Satt012 and Satt505. Utilizing SSR markers flanking the two QTLs, marker-assisted selection was performed in a second F-2:3 population of PI96354X Bossier. Results confirmed the effectiveness of marker-assisted selection to predict the Mi phenotypes. By screening the BC2F2 Population of Prichard (3)XG93-9009 we confirmed that selection for the minor QTL on LG-G with flanking SSR markers would enhance the resistance of lines containing, the major QTL (which is most-likely Rmil).