CLONING STRUCTURAL GENE SACB, WHICH CODES FOR EXOENZYME LEVANSUCRASE OF BACILLUS-SUBTILIS - EXPRESSION OF THE GENE IN ESCHERICHIA-COLI
CLONING STRUCTURAL GENE SACB, WHICH CODES FOR EXOENZYME LEVANSUCRASE OF BACILLUS-SUBTILIS - EXPRESSION OF THE GENE IN ESCHERICHIA-COLI
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DOI：
10.1128/jb.153.3.1424-1431.1983
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发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
HOCH, JA
中科院分区：
文献类型：
--
作者：
GAY, P;LECOQ, D;HOCH, JA
从B的文库中分离出一个带有编码外切酶果聚糖蔗糖酶的结构基因sac B的克隆。枯草杆菌DNA，克隆在噬菌体. charon 4A的嵌合DNA的转化活性的基础上。这个......该克隆还含有sacR和smo位点。将sacB-sacR区亚克隆到质粒pBR 325中，得到一个在大肠杆菌中指导果聚糖蔗糖酶合成的克隆。杆菌将编码分泌蛋白的核苷酸序列定位在克隆DNA的物理图谱上。
A clone bearing the structural gene sacB, coding for the exoenzyme levansucrase, was isolated from a library of B. subtilis DNA that was cloned in phage .lambda. charon 4A on the basis of the transforming activity of the chimeric DNA. This .lambda. clone also contained the sacR and smo loci. Subcloning the sacB-sacR region in plasmid pBR325 resulted in a clone which directed levansucrase synthesis in E. coli. The nucleotide sequence coding for the secreted protein was localized on the physical map of the cloned DNA.