Alternative Splicing May Not Be the Key to Proteome Complexity.
Alternative Splicing May Not Be the Key to Proteome Complexity.
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DOI：
10.1016/j.tibs.2016.08.008
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发表时间：
2017-03
影响因子：
13.8
通讯作者：
Valencia A
中科院分区：
文献类型：
--
作者：
Tress ML;Abascal F;Valencia A
选择性剪接通常被认为是细胞蛋白质多样性的主要来源。然而，尽管在RNAseq研究中经常检测到数以千计的选择性剪接转录本，基于可靠的大规模质谱学的蛋白质组学研究只发现了一小部分有注释的替代异构体。蛋白质组学实验中最明确的发现是，大多数人类基因只有一个主要的蛋白质异构体，而那些被识别的替代异构体往往是最具生物学可行性的：那些跨物种保守性最强的基因，以及那些不损害功能域的基因。事实上，大多数可供选择的外显子似乎没有受到选择压力，这表明绝大多数预测的可供选择的转录本甚至可能都不会被翻译成蛋白质。
Alternative splicing is commonly believed to be a major source of cellular protein diversity. However, although many thousands of alternatively spliced transcripts are routinely detected in RNAseq studies, reliable large-scale mass spectrometry-based proteomics studies identify only a small fraction of annotated alternative isoforms. The clearest finding from proteomics experiments is that most human genes have a single main protein isoform, while those alternative isoforms that are identified tend to be the most biologically plausible: those with the most cross-species conservation and those that do not compromise functional domains. Indeed, most alternative exons do not seem to be under selective pressure, suggesting that a large majority of predicted alternative transcripts may not even be translated into proteins.
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作者：
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