Designed transfer of specific immune responses with bone marrow transplantation.
Designed transfer of specific immune responses with bone marrow transplantation.
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设计通过骨髓移植转移特异性免疫反应。
DOI：
10.1172/jci112686
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Gale,RP
中科院分区：
文献类型：
--
作者：
Saxon,A;Mitsuyasu,R;Stevens,R;Champlin,RE;Kimata,H;Gale,RP
骨髓移植捐献者在骨髓捐献前6-7天接受破伤风/白喉类毒素免疫，以研究B细胞亚群在体液免疫重建中的作用。移植时在供体骨髓中检测到自发产生 IgG 抗破伤风和/或抗白喉类毒素的类淋巴母细胞 B 细胞。接受者的血清 IgG 抗破伤风和抗白喉类毒素水平迅速增加了 3-90 倍。三个供体/受体对中的抗白喉片段 A 抗体表现出光谱特性，表明供体反应的转移。移植后 64-154 天，三名接受者的重新免疫显示出与自发产生抗体的 B 细胞重新出现相关的 IgG 抗体反应，以及供体免疫反应的抗白喉片段 A 反应特征。这些观察结果扩展了对 B 细胞亚群作用的理解，并为骨髓移植中免疫的特异性调节提供了基础。
Bone marrow transplant donors were immunized with tetanus/diphtheria toxoids 6-7 d before bone marrow donation to investigate the role of B cell subpopulations in reconstitution of humoral immunity. Lymphoblastoid B cells spontaneously producing IgG antitetanus and/or antidiphtheria toxoid were detected in the donor marrows at the time of transplantation. Recipients rapidly demonstrated 3-90-fold increases in serum IgG antitetanus and antidiphtheria toxoid levels. Antidiphtheria fragment A antibody in three donor/recipient pairs demonstrated spectrotypic identity indicating transfer of the donors' response. Reimmunization of three recipients 64-154 d after transplant revealed an IgG antibody response associated with reappearance of spontaneous antibody-producing B cells and an antidiphtheria fragment A response characteristics of the donor's immune response. These observations extend the understanding of the role of B cell subpopulations and provide a basis for specific modulation of immunity in the setting of bone marrow transplantation.Images