Sensors of abiotic stress in Synechocystis
Sensors of abiotic stress in Synechocystis
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DOI：
10.1007/978-3-540-39402-0_5
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
K. Mikami;I. Suzuki;N. Murata
中科院分区：
文献类型：
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作者：
K. Mikami;I. Suzuki;N. Murata
组氨酸激酶的系统诱变与 DNA 微阵列技术相结合，使我们能够识别集胞藻中冷、高渗应激、氯化钠、磷酸盐和金属离子的传感器。 PCC 6803。他的激酶 33 (Hik33) 可感知冷应激和高渗应激，并在细胞暴露于各自的应激时调节不同组基因的表达。 Hik33、Hik34 和 Hik16 感知盐胁迫，它们各自调节一组不同基因的表达。 Hik7 已被确定为磷酸盐缺乏的传感器。此外，Hik27 (ManS) 和 Hik30 (NrsS) 分别检测锰离子缺乏和镍离子过量。六种蓝细菌菌株的基因组均包含 Hik33、Hik34 和 Hik7 基因，表明这三种组氨酸激酶可能对许多甚至所有蓝细菌的应激感知很重要。
Systematic mutagenesis of histidine kinases in combination with DNA microarray technology has allowed us to identify sensors for cold, hyperosmotic stress, sodium chloride, phosphate, and metal ions in Synechocystis sp. PCC 6803. His kinase 33 (Hik33) senses both cold and hyperosmotic stress and regulates the expression of different sets of genes when cells are exposed to each respective stress. Salt stress is perceived by Hik33, Hik34, and Hik16, each of which regulates the expression of a different set of genes. Hik7 has been identified as a sensor of phosphate deficiency. Moreover, manganese deficiency and an excess of nickel ions are sensed by Hik27 (ManS) and Hik30 (NrsS), respectively. The genomes of six strains of cyanobacteria all include genes for Hik33, Hik34, and Hik7, suggesting that these three histidine kinases might be important for the perception of stress in many or even all cyanobacteria.