Identification of regions on the fusion protein of human parainfluenza virus type 2 which are required for haemagglutinin-neuraminidase proteins to promote cell fusion
Identification of regions on the fusion protein of human parainfluenza virus type 2 which are required for haemagglutinin-neuraminidase proteins to promote cell fusion
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DOI：
10.1099/0022-1317-79-2-279
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发表时间：
1998-02-01
影响因子：
3.8
通讯作者：
Ito, Y
中科院分区：
文献类型：
--
作者：
Tsurudome, M;Ito, M;Ito, Y
在HeLa细胞中，我们已经证明了猴病毒41(SV-41)的融合蛋白(F)与人副流感病毒2型(PIV-2)的血凝素神经氨酸酶(HN)蛋白共表达时可诱导细胞融合，而PIV-2F蛋白不能诱导细胞与SV-41 HN蛋白的融合。对PIV-2和SV-41的F蛋白进行嵌合分析，确定了PIV-2 F蛋白上的两个区域(命名为M1和M2)，这两个区域中的任何一个是嵌合F蛋白与Muv HN蛋白显示融合活性所必需的，随后在PIV-2 F蛋白上又发现了两个额外的区域(P1和P2)，这两个区域都是嵌合F蛋白防止诱导细胞与SV-41 HN蛋白融合所必需的。因此，证明了给定的嵌合体F蛋白与M1或M2中的任何一个都含有P1和P2区，与PIV-2F蛋白一样，M2区位于PIV-2 F1胞外区的膜近端，而P1、M1和P2区聚集在胞外区的中部，PIV-2F蛋白上的这些区域可能参与了与HN蛋白的功能相互作用，这被认为是细胞融合的先决条件。
Using a plasmid expression system in HeLa cells, we have previously shown that the fusion (F) protein of simian virus 41 (SV-41) induces cell fusion when coexpressed with the haemagglutininneuraminidase (HN) protein of human parainfuenza virus type 2 (PIV-2), while the PIV-2 F protein does not induce cell fusion with the SV-41 HN protein, In the present study, we found that the PIV-2 F protein induced extensive cell fusion with the HN protein of mumps virus (MuV), whereas the SV-41 F protein did not, Chimaeric analyses of the F proteins of PIV-2 and SV-41 identified two regions (designated M1 and M2) on the PIV-2 F protein, either of which was necessary for chimaeric F proteins to show fusogenic activity with the MuV HN protein, Subsequently, two additional regions (P1 and P2) were identified on the PIV-2 F protein, both of which were necessary for chimaeric F proteins to prevent induction of cell fusion with the SV-41 HN protein, Consequently, it was proved that a given chimaeric F protein, harbouring regions P1 and P2 together with either of region M1 or M2, induced cell fusion specifically with HN proteins of PIV-2 and MuV, the same as the PIV-2 F protein, Region M2 was located at the membrane proximaI end of the PIV-2 F1 ectodomain, while regions P1, M1 and P2 clustered together in the middle of the ectodomain, These regions on the PIV-2 F protein may be involved in a putative functional interaction with HN proteins, which is considered to be a prerequisite for cell fusion.