Intrathecal morphine inhibits substance P release from mammalian spinal cord in vivo
Intrathecal morphine inhibits substance P release from mammalian spinal cord in vivo
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DOI：
10.1038/286155a0
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发表时间：
1980-07
期刊：
影响因子：
64.8
通讯作者：
T. L. Yaksh;T. Jessell;Rainer Gamse;Anne W. Mudge;Susan E. Leeman
中科院分区：
文献类型：
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作者：
T. L. Yaksh;T. Jessell;Rainer Gamse;Anne W. Mudge;Susan E. Leeman
与伤害性皮肤刺激的传递相关的小直径初级感觉神经元的中央末梢主要位于脊髓的浅层背角1 -3。其中一些神经元合成P物质（参考文献4），并将这种肽转运到它们的中枢和外周末端5 -7。P物质在钾离子去极化后从离体的背角释放8，9;在电刺激背根后从脊髓释放10，以及从培养物中生长的分离的感觉神经元释放11。P物质的离子电渗应用产生背角神经元的持久兴奋，其也被有害的皮肤刺激所兴奋12 -14。背角中的阿片受体的一个群体似乎位于初级传入末梢15 -16; P物质从初级感觉神经元的释放被体外阿片抑制8，11。然而，目前还没有直接的证据表明，P物质是释放的感觉神经元，在体内，激活伤害性传入。我们在这里报告，P物质样免疫反应性释放的哺乳动物脊髓，在体内，感觉神经元与辣椒素的化学刺激和高阈值外周传入的激活。此外，高强度刺激诱发的P物质释放被鞘内吗啡完全抑制。
The central terminals of small diameter primary sensory neurones associated with the transmission of noxious cutaneous stimuli are located predominantly in the superficial dorsal horn of the spinal cord1–3. Some of these neurones synthesize substance P (ref. 4) and transport this peptide to their central and peripheral terminals5–7. Substance P is released from the dorsal hornin vitrofollowing potassium depolarization8,9; from spinal cord after electrical stimulation of dorsal roots10and from dissociated sensory neurones grown in culture11. The iontophoretic application of substance P produces a long-lasting excitation of dorsal horn neurones that are also excited by noxious cutaneous stimuli12–14. One population of opiate receptors in the dorsal horn seems to be located on primary afferent terminals15–16; the release of substance P from primary sensory neurones is inhibited by opiatesin vitro8,11. At present, however, there is no direct evidence that substance P is released from sensory neurones,in vivo, following activation of nociceptive afferents. We report here that substance P-like immunoreactivity is released from the mammalian spinal cord,in vivo, following chemical stimulation of sensory neurones with capsaicin and by the activation of high threshold peripheral afferents. Furthermore, release of substance P evoked by high intensity stimuli is completely inhibited by intrathecal morphine.