Improved in vivo pancreatic islet function after prolonged in vitro islet culture
Improved in vivo pancreatic islet function after prolonged in vitro islet culture
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DOI：
10.1097/00007890-200112150-00005
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发表时间：
2001-12-15
期刊：
影响因子：
6.2
通讯作者：
Kotb, MY
中科院分区：
文献类型：
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作者：
Gaber, AO;Fraga, DW;Kotb, MY
背景胰岛的回收和保存困难阻碍了胰岛移植的进展。在以前的体外研究中，我们的实验室成功地证明，使用无血清培养基进行长期胰岛培养，可以使培养后的恢复率大于标准培养基获得的恢复率，而标准培养基具有持续的体外胰岛功能。本研究的目的是确定在改良的无血清培养基（M-SFM）中培养胰岛是否能维持体内功能。从12个尸体器官供体获得的胰腺中分离胰岛，并在M-SFM中培养长达2个月，在分离后1-3天内在-70 ℃下冷冻保存2个月，或在将其移植到非肥胖糖尿病-严重联合免疫缺陷小鼠（n=4-7/组/时间点）的肾囊下之前置于短期培养（3-5天）中。通过测量3-15个月内人胰岛素和C肽的产生来评估体内胰岛功能。结果。胰岛在M-SFM中延长培养1或2个月后，移植的胰岛保持其活力，并且在某些情况下，与从相同制备物移植的短期培养的胰岛相比，体内功能有所改善（P
Background. Difficulties with recovering and preserving pancreatic islets have hampered progress in islet transplantation. In previous in vitro studies, our laboratory successfully demonstrated that using serum-free medium for prolonged pancreatic islet culture allows postculture recovery ratios greater than those obtained,with standard media with sustained in vitro islet function. The goal of this study was to determine whether culturing of islets in a modified serum-free medium (M-SFM) would sustain function in vivo.Methods. Islets were isolated from pancreata procured from 12 cadaveric organ donors and cultured in the M-SFM for up to 2 months, cryopreserved at -70 degreesC within 1-3 days of isolation for 2 months, or placed in short-term culture (3-5 days) before their transplantation under the kidney capsule of nonobese diabetic-severe combined immunodeficient mice (n=4-7 per group/time point). In vivo islet function was assessed by measuring the production of human insulin and C-peptide over a period of 3-15 months.Results. After extended culture of islets in M-SFM for 1 or 2 months, transplanted islets maintained their viability, and in some instances in vivo function improved when compared with short-term cultured islets transplanted from the same preparation (P