Beneficial effects of green tea catechin on massive hepatectomy model in rats
Beneficial effects of green tea catechin on massive hepatectomy model in rats
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DOI：
10.1007/s00535-013-0799-9
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发表时间：
2014-04-01
影响因子：
6.3
通讯作者：
Shimada, Mitsuo
中科院分区：
文献类型：
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作者：
Saito, Yu;Mori, Hiroki;Shimada, Mitsuo
绿色儿茶素，特别是表没食子儿茶素没食子酸酯（epigallocatechin gallate，EGCG），是一种活性氧清除剂，通过调节转录因子和酶的活性而发挥抗氧化剂的作用。Wistar大鼠行90%肝切除术，分为GTE治疗组（Hx，n = 25）和GTE治疗组（Hx，n = 25），分别于术后1、3、7和14天处死。从术前第7天开始，所有大鼠均可自由饮用补充或不补充GTE的饮用水。肝再生、肝诱导型一氧化氮合酶（iNOS）、抗氧化酶[超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）]和炎症标志物[环氧合酶-2（考克斯-2）、核因子κ B（NF κ B）、肿瘤坏死因子-α（TNF-α）]进行了研究。（P < 0.01），增殖细胞核抗原标记指数与Hx组相比，GTEHx组第1天的磷酸化细胞外信号调节激酶1/2（p <0.05）和磷酸化细胞外信号调节激酶1/2（p < 0.05）显著增加。GTEHx组第1天肝脏iNOS水平显著降低（p < 0.01）。GTEHx组第1天肝脏SOD、CAT和GSH-Px水平显著升高（SOD：p < 0.01，CAT和GSH-Px：p < 0.05）。相反，在GTEHx组中，第1天考克斯-2、NF κ B和TNF-α水平显著降低（考克斯-2：p < 0.01，NF κ B和TNF-α：p < 0.05）JTE预处理通过抗氧化和抗炎作用刺激肝再生并改善大面积肝切除术后的肝损伤。绿色茶儿茶素可能具有减轻大肝切除术后早期肝功能障碍的潜力。
Green tea catechin, especially epigallocatechin gallate (EGCG), is a well-known scavenger of reactive oxygen species and it may also function as an antioxidant through modulation of transcriptional factors and enzyme activities.Green tea extract (GTE(A (R))) which contained numerous EGCG was used. Wistar rats were performed 90 % hepatectomy and classified into 2 groups with (GTEHx, n = 25) or without GTE treatment (Hx, n = 25) and sacrificed at 1, 3, 7 and 14 days after operations. All rats had free access to drinking water supplemented with or without GTE from the 7th pre-operative day. Liver regeneration, hepatic inducible nitric oxide synthase (iNOS), anti-oxidative enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px)] and inflammatory markers [cyclooxygenase-2 (COX-2), nuclear factor kappa B (NF kappa B), tumor necrosis factor-alpha (TNF-alpha)] were investigated.The liver weight to body weight ratio (p < 0.01), proliferating cell nuclear antigen labeling index (p < 0.05) and phosphorylated extracellular signal-regulated kinase 1/2 (p < 0.05) at day 1 in the GTEHx group significantly increased compared to the Hx group. Hepatic iNOS levels at day 1 significantly decreased (p < 0.01) in the GTEHx group. Hepatic SOD, CAT and GSH-Px levels at day 1 significantly increased (SOD: p < 0.01, CAT and GSH-Px: p < 0.05) in the GTEHx group. In contrast, COX-2, NF kappa B and TNF-alpha levels at day 1 significantly decreased (COX-2: p < 0.01, NF kappa B and TNF-alpha: p < 0.05) in the GTEHx group.GTE pretreatment stimulated liver regeneration and improved liver damage after massive hepatectomy through anti-oxidative and anti-inflammatory effects. Green tea catechin might have the potential to attenuate liver dysfunction in early stage after massive hepatectomy.