Diffusible, membrane-bound, and extracellular matrix factors from olfactory ensheathing cells have different effects on the self-renewing and differentiating properties of neural stem cells
Diffusible, membrane-bound, and extracellular matrix factors from olfactory ensheathing cells have different effects on the self-renewing and differentiating properties of neural stem cells
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DOI：
10.1016/j.brainres.2010.08.063
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发表时间：
2010-11-04
期刊：
影响因子：
2.9
通讯作者：
He, Cheng
中科院分区：
文献类型：
--
作者：
Cao, Li;Mu, Lifang;He, Cheng
嗅鞘细胞（OECs）移植是促进中枢神经系统（CNS）再生的一种很有前途的策略。然而，很少有人知道移植的嗅鞘细胞的自我更新，神经发生，和少突神经干细胞（NSCs），这是已知的，在受损的中枢神经系统组织的修复中发挥了非常重要的作用的影响。在这项研究中，我们研究了嗅鞘细胞的扩散，膜结合，和细胞外基质因子对神经干细胞的自我更新和分化特性的影响。我们发现，从培养的嗅鞘细胞的扩散因子促进自我更新，而从嗅鞘细胞的细胞外基质分子增加神经发生和少突神经干细胞。此外，我们证明，直接共培养嗅鞘细胞和神经干细胞不仅抑制自我更新，但也神经发生和少突神经干细胞。我们提出了三种模型移植的嗅鞘细胞和内源性神经干细胞之间的相互作用。我们的研究结果为OEC促进中枢神经系统修复的能力提供了新的见解，并指出了操纵这些细胞以增强其恢复能力的潜在目标。(C)2010 Elsevier B.V.保留所有权利。
Transplantation of olfactory ensheathing cells (OECs) has been a promising strategy in enhancing central nervous system (CNS) regeneration. However, little is known about the effects of transplanted OECs on the self-renewal, neurogenesis, and oligodendrogenesis of neural stem cells (NSCs), which are known to play a very important role in the repair of damaged CNS tissue. In this study, we investigated the influence of diffusible, membrane-bound, and extracellular matrix factors from OECs on the self-renewal and differentiation properties of NSCs. We found that diffusible factors from cultured OECs promoted self-renewal, whereas the extracellular matrix molecules from OECs increased neurogenesis and oligodendrogenesis of NSCs. Furthermore, we demonstrated that directly coculturing OECs and NSCs inhibited not only self-renewal but also neurogenesis and oligodendrogenesis of NSCs. We propose three models for the interaction between transplanted OECs and endogenous NSCs. Our findings provide new insight into the ability of OECs to promote CNS repair and also indicate potential targets for manipulation of these cells to enhance their restorative ability. (C) 2010 Elsevier B.V. All rights reserved.