The Bacillus subtilis essential gene dgkB is dispensable in mutants with defective lipoteichoic acid synthesis.
The Bacillus subtilis essential gene dgkB is dispensable in mutants with defective lipoteichoic acid synthesis.
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枯草芽孢杆菌必需基因 dgkB 在脂磷壁酸合成缺陷的突变体中是可有可无的。
DOI：
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发表时间：
2011
影响因子：
1.1
通讯作者：
Kouji Matsumoto
中科院分区：
文献类型：
--
作者：
S. Matsuoka;Michihiro Hashimoto;Yusuke Kamiya;T. Miyazawa;Kazuki Ishikawa;H. Hara;Kouji Matsumoto
dgkB 基因对于枯草芽孢杆菌的生长至关重要。它编码二酰甘油 (DG) 激酶，将 DG 转化为磷脂酸，将其重新引入磷脂合成途径。置于可调节启动子下的 dgkB 基因的抑制会导致 DG 积累并导致致死。 DG 是脂磷壁酸 (LTA) 合成的副产品，脂磷壁酸是细胞膜的聚阴离子成分。枯草芽孢杆菌通过将磷脂酰甘油 (PG) 的甘油磷酸部分聚合到糖脂膜锚上，并释放 PG 的 DG 部分来合成 LTA。枯草芽孢杆菌有四个与金黄色葡萄球菌ltaS同源的基因，其编码LTA合酶。 yflE 和 yfnI 两个基因中的一个或两个基因的破坏抑制了 dgkB 抑制引起的致死率，这两个基因的产物与金黄色葡萄球菌 LtaS 在四个同源物中表现出更高的同源性。在 dgkB 抑制的细胞中，DG 累积至总脂质的 43 ± 3%，大约是野生型细胞含量 (13 ± 1%) 的三倍。 dgkB 抑制细胞中 yfnI 的破坏将 DG 含量降低至 15 ± 2%，但 yflE 破坏却没有（42 ± 1%）；这可能是由于 yflE 破坏的细胞中 YfnI 有效合成 LTA。进一步引入ugtP的破坏等位基因，编码消耗DG作为底物的糖脂合酶，部分降低了yflE破坏菌株的集落形成能力。破坏的 dgkB 等位基因成功地引入到 yflE 和 yfnI 之一或两者破坏的菌株中，表明必需基因 dgkB 在 LTA 合成缺陷的突变体中是可有可无的。
The dgkB gene is essential for the growth of Bacillus subtilis. It encodes a diacylglycerol (DG) kinase that converts DG to phosphatidic acid to reintroduce it into the phospholipid synthesis pathway. Repression of the dgkB gene placed under a regulatable promoter causes accumulation of DG and leads to lethality. DG is formed as a byproduct of the synthesis of lipoteichoic acid (LTA), a polyanionic component of the cell envelope. B. subtilis synthesizes LTA by polymerizing the glycerophosphate moiety of phosphatidylglycerol (PG) onto a glucolipid membrane anchor, and releasing the DG moiety of PG. B. subtilis has four genes homologous to Staphylococcus aureus ltaS, which encodes LTA synthase. Disruption of either or both of two genes, yflE and yfnI, whose products show higher homology with S. aureus LtaS among the four homologues, suppressed the lethality caused by dgkB repression. In cells with dgkB repression, DG was accumulated to 43 ± 3% of total lipids, about three times the content of wild type cells (13 ± 1%). Disruption of yfnI in the dgkB-repressed cells reduced the DG content to 15 ± 2%, but yflE-disruption did not (42 ± 1%); this was probably due to efficient LTA synthesis by YfnI in the yflE-disrupted cells. Further introduction of a disrupted allele of ugtP, encoding glucolipid synthase that consumes DG as a substrate, partially lowered the colony forming capacity in strains with yflE-disruption. A disrupted dgkB allele was successfully introduced into strains disrupted for either or both of yflE and yfnI, indicating that the essential gene dgkB is dispensable in mutants defective in LTA synthesis.
大肠杆菌中膜源性寡糖合成的调节。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bohin,JP;Kennedy,EP
通讯作者：
Kennedy,EP
影响因子：
2.9
作者：
Sanders, CR;Czerski, L;Smith, SO
通讯作者：
Smith, SO