Establishment of a Rabbit Model for Keratoconjunctivitis Sicca
Establishment of a Rabbit Model for Keratoconjunctivitis Sicca
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兔干燥性角结膜炎模型的建立
DOI：
10.1097/ico.0b013e3181f1b0fc
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发表时间：
2011-09-01
期刊：
影响因子：
2.8
通讯作者：
Yu, Guang-yan
中科院分区：
文献类型：
--
作者：
Chen, Zhi-yuan;Liang, Qing-feng;Yu, Guang-yan
目的：目的：建立干燥性角结膜炎（keratoconjunctivitissicca，KCS）兔模型，研究自体颌下腺移植治疗重症KCS的效果。三氯乙酸治疗组手术切除泪腺、哈氏腺和瞬膜，用50%三氯乙酸擦拭眼睑和球结膜。在非三氯乙酸治疗组中，手术切除泪腺、哈氏腺和瞬膜。在术前和术后1、2、3和4个月进行Schirmer试验。术后每月对角膜的虎红染色和荧光素染色密度进行评分。在4个月时，从手术眼和对照眼中取出角膜和球结膜进行组织病理学检查。结果：与术前相比，两组术后泪液分泌量均明显减少，然后逐渐增加。两组手术眼角膜玫瑰红和荧光素染色评分均高于对照眼，结膜杯状细胞密度低于对照眼，但两组手术眼间差异无统计学意义。结论：两种方法均能建立一种新的兔KCS模型。通过手术切除泪腺、哈氏腺和瞬膜，可以减少泪液产生的实验性KCS。不必使用三氯乙酸灼伤结膜。我们改良的切口能更好地暴露手术野。
Purpose: To establish a rabbit model for keratoconjunctivitis sicca (KCS) to study autologous submandibular gland transfer for treating severe KCS.Methods: In 2 groups of 10 rabbits, left eyes were operated and right eyes were controls. In the trichloroacetic acid-treated group, the lacrimal and harderian glands and nictitating membrane were removed surgically; the palpebral and bulbar conjunctiva were swabbed with 50% trichloroacetic acid. In the non-trichloroacetic acid-treated group, the lacrimal and harderian glands and nictitating membrane were surgically removed. The Schirmer test was performed preoperatively and 1, 2, 3, and 4 months postoperatively. Corneal densities of rose bengal and fluorescein staining were scored every month postoperatively. At 4 months, the cornea and bulbar conjunctiva were removed from operated and control eyes for histopathology. The upper bulbar conjunctiva was used to determine goblet cell density.Results: Compared with preoperative conditions, tear secretion of operated eyes significantly decreased in both groups postoperatively, then gradually increased. Scores for corneal rose bengal and fluorescein staining were higher and conjunctival goblet cell density was lower in the operated eyes than in control right eyes in both groups, but no significant difference was found between the operated eyes of the two groups. Inflammatory histopathologic changes of the cornea and conjunctivawere not found in either of the eyes in the two groups.Conclusions: A new rabbit model for KCS could be created by either of these methods. Experimental KCS with reduction of tear production was possible with surgical ablation of the lacrimal and harderian glands and nictitating membrane. It is unnecessary to apply trichloroacetic acid to burn the conjunctiva. Our modified incision better exposed the surgical field.