Self-incompatibility alleles control a low molecular weight, basic protein in pistils of Petunia hybrida
Self-incompatibility alleles control a low molecular weight, basic protein in pistils of Petunia hybrida
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自交不亲和性等位基因控制矮牵牛雌蕊中的低分子量碱性蛋白质
DOI：
10.1007/bf00276423
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发表时间：
1986
影响因子：
5.4
通讯作者：
J. Jackson
中科院分区：
文献类型：
--
作者：
R. Kamboj;J. Jackson
用凝胶电泳法对矮牵牛与不同S等位基因配对的几个无性系雌蕊提取的蛋白质进行了分析。雌蕊的主要蛋白质是一种相对低分子量的碱性糖蛋白，其性质与S基因型的差异很小。对于每个S等位基因，我们能够为这个推测的S蛋白指定一个特定的相对分子质量(范围为27,000到33,000)和等电点(范围为8.3到8.7)。在双向凝胶电泳法上，S纯合子雌蕊只显示一种蛋白质，而S基因杂合植株的雌蕊则显示两种蛋白质。没有证据表明在花粉提取液中存在这种可能的S蛋白。
Proteins extracted from the pistils of several clones ofPetunia hybridacarrying differing pairs of S alleles were examined by gel electrophoresis. The major protein of pistils, a basic glycoprotein of relatively low molecular weight, showed properties which varied in a simple manner with the S genotype. For each S allele we were able to assign a specific molecular weight (ranging from 27,000 to 33,000) and isoelectric point (in the range 8.3 to 8.7) for this putative S protein. Pistils homozygous at the S locus showed only one major protein on two-dimensional gel electrophoresis, while pistils from plants heterozygous at the S locus showed two. No evidence was obtained for the presence of this putative S protein in pollen extracts.