Acquisition of non-MHC restricted cytotoxic function by IL 2 activated thymocytes with an "immature" antigenic phenotype.
Acquisition of non-MHC restricted cytotoxic function by IL 2 activated thymocytes with an "immature" antigenic phenotype.
复制标题
IL 2 获得非 MHC 限制的细胞毒功能，激活具有“未成熟”抗原表型的胸腺细胞。
作者：
J. Phillips;L. Lanier
人胸腺细胞在白细胞介素2（IL 2）中培养可产生细胞毒性T淋巴细胞（CTL），其杀伤肿瘤细胞的靶细胞而不受主要组织相容性复合体（MHC）的限制。胸腺非MHC限制性CTL表达Leu-19抗原，但由缺乏Leu-19表达的胸腺前体细胞产生。相反，在IL 2中短期培养去除Leu-19+淋巴细胞的外周血淋巴细胞不会导致产生细胞毒活性。IL 2是产生细胞毒性胸腺细胞和诱导Leu-19抗原表达所必需和充分的。胸腺非MHC限制性CTL由表达CD 1的前体细胞产生，CD 1是存在于大多数胸腺细胞上的抗原。此外，在IL 2培养的具有“未成熟”抗原表型（即CD 1+和CD 4+、CD 8+）的胸腺细胞群中检测到细胞毒活性。在随后的培养中，胸腺非MHC限制性CTL失去了CD 1的表达，并产生了与外周血非MHC限制性CTL相似的抗原表型，这表明外周非MHC限制性CTL可能起源于这些胸腺前体。
Culture of human thymocytes in interleukin 2 (IL 2) results in the generation of cytotoxic T lymphocytes (CTL) that kill tumor cell targets without major histocompatibility complex (MHC) restriction. Thymic non-MHC restricted CTL expressed Leu-19 antigen, but were generated from thymic precursor cells that lacked expression of Leu-19. In contrast, short term culture in Il 2 of peripheral blood lymphocytes depleted of Leu-19+ lymphocytes did not result in the generation of cytotoxic activity. IL 2 was necessary and sufficient for the generation of cytotoxic thymocytes and induction of Leu-19 antigen expression. Thymic non-MHC restricted CTL were generated from precursor cells expressing CD1, an antigen present on the majority of thymocytes. Furthermore, cytotoxic activity was detected in IL 2 cultured thymocyte populations with an "immature" antigenic phenotype, i.e. CD1+ and CD4+, CD8+. Upon subsequent culture, thymic non-MHC restricted CTL lost expression of CD1, and developed an antigenic phenotype similar to peripheral blood non-MHC-restricted CTL, suggesting that peripheral non-MHC-restricted CTL may originate from these thymic precursors.