An enhanced isothermal amplification assay for viral detection.
An enhanced isothermal amplification assay for viral detection.
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用于病毒检测的增强的等温扩增测定法。
DOI：
10.1038/s41467-020-19258-y
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发表时间：
2020-11-20
影响因子：
16.6
通讯作者：
Springer M
中科院分区：
文献类型：
--
作者：
Qian J;Boswell SA;Chidley C;Lu ZX;Pettit ME;Gaudio BL;Fajnzylber JM;Ingram RT;Ward RH;Li JZ;Springer M
快速、廉价、可靠的诊断对控制传染病的传播至关重要。目前的诊断技术是高度敏感和特异的，但速度慢，需要昂贵的设备。在这里，我们报告了一种基于增强型重组酶聚合酶扩增（eRPA）反应的SARS-CoV-2分子诊断测试的发展。eRPA对患者样本的检测限低至5个病毒拷贝，需要最少的仪器，并且具有高度可扩展性和廉价性。eRPA不与其他常见冠状病毒发生交叉反应，不需要RNA纯化，从样本采集到结果得出需要约45分钟。eRPA代表了家庭SARS-CoV-2检测的第一步，可以在基因组序列可用的几天内适应未来的病毒。目前最先进的传染病诊断方法很敏感，但需要大量设备。在这里，作者开发了一种增强的SARS-CoV-2重组酶聚合酶扩增反应，可以用最少的设备进行廉价和快速的检测。
Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. Here we report the development of a molecular diagnostic test for SARS-CoV-2 based on an enhanced recombinase polymerase amplification (eRPA) reaction. eRPA has a detection limit on patient samples down to 5 viral copies, requires minimal instrumentation, and is highly scalable and inexpensive. eRPA does not cross-react with other common coronaviruses, does not require RNA purification, and takes ~45 min from sample collection to results. eRPA represents a first step toward at-home SARS-CoV-2 detection and can be adapted to future viruses within days of genomic sequence availability. Current state-of-the-art diagnostics for infectious diseases are sensitive but require extensive equipment. Here the authors develop an enhanced recombinase polymerase amplification reaction for SARS-CoV-2 that allows for inexpensive and rapid testing with minimal equipment.