Local injection of hepatocyte growth factor scatter factor (HGF/SF) alters cyclic growth of murine hair follicles
Local injection of hepatocyte growth factor scatter factor (HGF/SF) alters cyclic growth of murine hair follicles
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DOI：
10.1046/j.1523-1747.1998.00144.x
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发表时间：
1998-04-01
影响因子：
6.5
通讯作者：
Ogawa, H
中科院分区：
文献类型：
--
作者：
Jindo, T;Tsuboi, R;Ogawa, H
肝细胞生长因子/分散因子（HGF/SF）最近已被证明可以刺激体内小鼠触须毛囊的生长。在这项研究中，我们分析了皮肤注射重组人HGF/SF对处于不同毛发周期阶段的小鼠毛囊生长的影响。5只雄性新生小鼠、5只处于第二生长期的雄性小鼠和5只处于第二休止期的雄性小鼠被给予背部皮内注射溶于0.1%白蛋白磷酸盐缓冲盐水中的1 μ g HGF/SF，每天一次，连续5天或7天，然后在第7天或第10天处死，使用三个参数通过光度计和组织学评价毛囊生长：切除皮肤的反面的皮肤颜色、皮肤厚度和毛囊组织占据的面积，注射HGF/SF的新生小鼠皮肤的毛囊在组织学上比注射0.1%磷酸白蛋白的小鼠的毛囊更长更大。注射缓冲盐水的皮肤。在第二生长期接受HGF/SF注射的小鼠，仅在注射部位在10 d后保留生长期毛囊，表明HGF/SF延迟了从生长期到休止期的过渡。注射HGF/SF的休止期小鼠的皮肤在真皮中的毛囊组织中具有显著增加，这表明HGF/SF具有温和的生长期诱导活性。此外，从HGF/SF注射部位拔出的20根毛发的精确测量显示在所有上述实验中轻度毛发伸长。这些结果表明，HGF/SF作为旁分泌因子，改变小鼠周期性毛发生长。
Hepatocyte growth factor/scatter factor (HGF/SF) has recently been shown to stimulate the hair follicle growth of mouse vibrissae in vivo. In this study, we analyzed the effect of cutaneous injections of recombinant human HGF/SF on hair follicle growth using mice in different hair cycle stages, Five male newborn mice, five male mice in second anagen, and five male mice in second telogen were administered a dorsal intradermal injection of 1 mu g HGF/SF dissolved in 0.1% albumin-phosphate-buffered saline once daily for five or seven consecutive days, and then sacrificed on days 7 or 10, Hair follicle growth was evaluated photometrically and histologically using three parameters: the skin color of the reverse side of the resected skin, the skin thickness, and the area occupied by hair follicle tissue, The HGF/SF injected skin of newborn mice had hair follicles that were histologically longer and larger than those of the 0.1% albumin-phosphate-buffered saline injected skin. Mice that had received HGF/SF injection in second anagen, retained anagen hair follicles after 10 d only at the injection site, suggesting that HGF/SF delayed the transition from anagen to telogen. The HGF/SF injected skin of telogen mice had a significant increase in hair follicle tissue in the dermis, suggesting a mild anagen inducible activity by HGF/SF. Furthermore, precise measurements of the 20 hairs plucked from the HGF/SF injection sites revealed mild hair elongation in all the aforementioned experiments. These results imply that HGF/SF acts as a paracrine factor that alters cyclic hair growth of mice.