Metabolic profile of chiral cobalt oxide nanoparticles in vitro and in vivo
Metabolic profile of chiral cobalt oxide nanoparticles in vitro and in vivo
复制标题
手性氧化钴纳米粒子的体外和体内代谢特征
DOI：
10.1007/s12274-020-3250-6
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发表时间：
2021
期刊：
影响因子：
9.9
通讯作者：
Kuang Hua
中科院分区：
文献类型：
--
作者：
Li Si;Xu Liwei;Lu Meiru;Sun Maozhong;Xu Liguang;Hao Changlong;Wu Xiaoling;Xu Chuanlai;Kuang Hua
在本研究中，我们研究了D-或L-半胱氨酸稳定的手性氧化钴纳米粒子(Co3O4 NPs)在体内的行为。手性Co3O4纳米粒在实验剂量范围内未表现出明显的细胞毒性。D-NPs和L-NPs均通过小窝依赖的内吞作用被树突状细胞内化，且L-NPs比D-NPs更快进入细胞。值得注意的是，代谢分析表明，手性L纳米粒对肝脏胆汁酸水平的影响与D-纳米粒相同，这归因于手性纳米粒诱导的法尼醇X受体(FXR)的数量几乎相同。这项研究表明，低手性纳米材料在生理条件下不会影响代谢和动力学，即使它们的转运存在一定的差异。
In this study, we investigated the in vivo behaviors of chiral cobalt oxide nanoparticles (Co 3 O 4 NPs) stabilized with D- or L-cysteine (denoted D- or L-NPs, respectively), as representative chiral metal oxide NPs. Chiral Co 3 O 4 NPs exerted no observable cytotoxicity within the tested dose range. Both D-NPs and L-NPs were internalized by dendritic cells through caveola-dependent endocytosis, and L-NPs entered the cells faster than D-NPs. Significantly, a metabolic analysis indicated that chiral L-NPs had the same effect on the level of bile acids in the liver as D-NPs, which is attributed to the almost equal amount of farnesoid X receptor (FXR) induced by the chiral NPs. This study suggests that low chiroptical activity nanomaterials would not affect the metabolism and kinetics under physiological conditions even if there is some difference in their transport.