Differentiation of mouse hepatitis virus genotypes by restriction fragment length polymorphism analysis
Differentiation of mouse hepatitis virus genotypes by restriction fragment length polymorphism analysis
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DOI：
10.1258/0023677052886574
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发表时间：
2005-01-01
影响因子：
2.4
通讯作者：
Ohsugi, T
中科院分区：
文献类型：
--
作者：
Nakamura, N;Yoshizumi, S;Ohsugi, T
用逆转录聚合酶链式反应(RT-PCR)扩增小鼠肝炎病毒(MHV)核衣壳(N)基因，成功地从2000-2003年日本动物设施饲养的小鼠粪便中检测到36株病毒。随后利用Ace I、Alu I、EcoR I和Mbo I酶进行的限制性片段长度多态性(RFLP)分析表明，这些毒株可分为5个不同的亚组，并且从密切相关的小鼠设施中检测到相同的MHV毒株。此外，来自同一设施的菌株显示出相同的RFLP图谱，与检测年份无关，但这种图谱在不同地点之间有所不同。本研究表明，RFLP分析是一种快速、有效的MHV毒株鉴别方法。
Reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of the nucleocapsid (N) gene of mouse hepatitis virus (MHV) successfully detected 36 strains from the faeces of mice that had been housed in animal facilities in Japan from 2000 to 2003. Subsequent restriction fragment length polymorphism (RFLP) analysis, using the enzymes Ace I, Alu I, EcoR I and Mbo I, demonstrated that these strains could be divided into five distinct subgroups and that the same MHV strains were detected from closely related mouse facilities. Furthermore, strains from the same facility showed the same RFLP pattern, irrespective of the year of detection, but this pattern varied between different locations. This study shows that RFLP analyses are a rapid and useful method for differentiation of MHV strains.