Bacteria Use Type IV Pili to Walk Upright and Detach from Surfaces
Bacteria Use Type IV Pili to Walk Upright and Detach from Surfaces
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DOI：
10.1126/science.1194238
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发表时间：
2010-10-08
期刊：
影响因子：
56.9
通讯作者：
Wong, Gerard C. L.
中科院分区：
文献类型：
--
作者：
Gibiansky, Maxsim L.;Conrad, Jacinta C.;Wong, Gerard C. L.
细菌生物膜是一种结构化的多细胞生物群落，广泛存在于各种感染中。了解自由游动的细菌如何在表面附近适应它们的运动机制对于理解浮游生物和生物膜表型之间的转变至关重要。通过将显微镜电影翻译成可搜索的细菌行为数据库，我们确定了铜绿假单胞菌表面运动的基本IV型菌毛驱动机制，这些机制涉及不同的觅食策略。细菌直立并“行走”，其轨迹针对二维表面探索进行了优化。垂直方向有利于表面分离，并可能影响生物膜形态。
Bacterial biofilms are structured multicellular communities involved in a broad range of infections. Knowing how free-swimming bacteria adapt their motility mechanisms near surfaces is crucial for understanding the transition between planktonic and biofilm phenotypes. By translating microscopy movies into searchable databases of bacterial behavior, we identified fundamental type IV pili-driven mechanisms for Pseudomonas aeruginosa surface motility involved in distinct foraging strategies. Bacteria stood upright and "walked" with trajectories optimized for two-dimensional surface exploration. Vertical orientation facilitated surface detachment and could influence biofilm morphology.