Binding of Dipeptides to Fatty Acid Membranes Explains Their Colocalization in Protocells but Does Not Select for Them Relative to Unjoined Amino Acids.
Binding of Dipeptides to Fatty Acid Membranes Explains Their Colocalization in Protocells but Does Not Select for Them Relative to Unjoined Amino Acids.
复制标题
二肽与脂肪酸膜的结合解释了它们在原细胞中共存的原因，但并不是相对于未连接的氨基酸而选择它们。
DOI：
10.1021/acs.jpcb.1c01485
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发表时间：
2021-07-29
期刊：
影响因子：
--
通讯作者：
Keller SL
中科院分区：
文献类型：
--
作者：
Xue M;Black RA;Cohen ZR;Roehrich A;Drobny GP;Keller SL
由两个氨基酸通过肽键连接而成的二肽已被证明具有催化功能。这一发现引出了与生命起源有关的基本问题。肽是如何与最初的原始细胞共定位的？哪些结构特征决定了氨基酸和肽与膜的结合？二肽与原始细胞膜的结合是否推动了分子进化，从而有利于二肽而不是单个氨基酸？使用脉冲场梯度核磁共振（PFG-NMR），我们发现几种益生元氨基酸和二肽结合到益生元膜。对于氨基酸，侧链和羧基有助于相互作用。对于二肽，结合的程度通常小于组成氨基酸的结合程度，这意味着驱动分子进化需要其他机制。然而，我们的结果是一致的方案，其中的生物聚合物的积木共定位与原始细胞出现之前的RNA和蛋白质。
Dipeptides, which consist of two amino acids joined by a peptide bond, have been shown to have catalytic function. This observation leads to fundamental questions relevant to the origins of life. How could peptides have become co-localized with the first protocells? Which structural features would have determined the association of amino acids and peptides with membranes? Could the association of dipeptides with protocell membranes have driven molecular evolution, favoring dipeptides over individual amino acids? Using pulsed-field gradient nuclear magnetic resonance (PFG-NMR), we find that several prebiotic amino acids and dipeptides bind to prebiotic membranes. For amino acids, the sidechains and carboxylate contribute to the interaction. For dipeptides, the extent of binding is generally less than that of the constituent amino acids, implying that other mechanisms would be necessary to drive molecular evolution. Nevertheless, our results are consistent with a scheme in which the building blocks of the biological polymers co-localized with protocells prior to the emergence of RNA and proteins.
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影响因子：
5.5
作者：
Blocher, M;Liu, DJ;Luisi, PL
通讯作者：
Luisi, PL
DOI：
10.3390/life6030033
发表时间：
2016-08-11
期刊：
Life (Basel, Switzerland)
影响因子：
--
作者：
Black RA;Blosser MC
通讯作者：
Blosser MC
影响因子：
2
作者：
Pizzarello, Sandra;Weber, Arthur L.
通讯作者：
Weber, Arthur L.
影响因子：
2.4
作者：
Einstein, A
通讯作者：
Einstein, A
影响因子：
16.6
作者：
Rodriguez-Garcia, Marc;Surman, Andrew J.;Cronin, Leroy
通讯作者：
Cronin, Leroy