Optimization of the hyphenation between capillary zone electrophoresis and inductively coupled plasma mass spectrometry for the measurement of As-, Sb-, Se- and Te-species, applicable to soil extracts
Optimization of the hyphenation between capillary zone electrophoresis and inductively coupled plasma mass spectrometry for the measurement of As-, Sb-, Se- and Te-species, applicable to soil extracts
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DOI：
10.1016/s0584-8547(01)00365-2
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发表时间：
2002-01-09
影响因子：
3.3
通讯作者：
Potin-Gautier, M
中科院分区：
文献类型：
--
作者：
Casiot, C;Donard, OFX;Potin-Gautier, M
研究了毛细管区带电泳（CZE）与电感耦合等离子体质谱（ICP-MS）联用同时测定环境中类金属的优化条件。砷（亚砷酸盐，砷酸盐，单甲基胂酸，二甲基胂酸），硒（亚硒酸盐，硒酸盐，硒代亚硒酸盐。硒代胱氨酸）、锑（锑酸盐）和碲（亚碲酸盐）。碲酸盐）物种同时使用75 μ mi.d.使用铬酸盐或磷酸盐电解质的熔融石英毛细管。测试了不同雾化器在检测器中的引入。研究了V型槽雾化器（Babington）和两种同心微雾化器（MCN-100和MicroMist），以提高分辨率。灵敏度和再现性。CE-ICP-MS接口操作参数的优化讨论了每个雾化器接口组合，并特别注意雾化器内的毛细管的位置。详细研究了喷雾器气液鞘流速对电泳分辨率和峰宽的影响。使用MicroMist雾化器获得了最佳的分析性能特征。使用该喷雾器的检测限范围为6至58 μ g l（-1），取决于使用压力进样研究的物种，对于大多数使用电迁移进样的物种，检测限低于1 μ g l（-1）。对土壤提取物的分析表明，有可能对真实的样品进行这项技术。(C)2002 Elsevier Science B. V.保留所有权利。
The optimization of the hyphenation between capillary zone electrophoresis (CZE) and inductively coupled plasma mass spectrometry (ICP-MS) was studied for the simultaneous determination of metalloid species in the environment. Arsenic (arsenite, arsenate, monomethylarsonic acid, dimethylarsinic acid), selenium (selenite, selenate, selenomethionine. selenocystine), antimony (antimonate) and tellurium (tellurite. tellurate) species were simultaneously separated using a 75-mum i.d. fused silica capillary using either a chromate or a phosphate electrolyte. Different nebulizers were tested for introduction in the detector. A V-groove nebulizer (the Babington) and two concentric micronebulizers (the MCN-100 and the MicroMist) were studied in order to improve resolution. sensitivity and reproducibility. The optimization of CE-ICP-MS interface operating parameters is discussed for each nebulizer-interface combination, and special attention is given to the position of the capillary inside the nebulizer. Different nebulizer gas and liquid sheath flow rates were studied in detail and they hardly affect electrophoretic resolution and peak width. The best analytical performance characteristics were obtained with the MicroMist nebulizer. Detection limits with this nebulizer were found to range between 6 and 58 mug l(-1) depending on the species investigated using pressure injection and below 1 mug l(-1) for most of the species with electromigrative injection. Analysis of soil extracts showed that it was possible to carry out this technique on real samples. (C) 2002 Elsevier Science B.V. All rights reserved.