Identification and structural characterization of two genes encoding glutamate transporter homologues differently expressed in the nervous system of Drosophila melanogaster
Identification and structural characterization of two genes encoding glutamate transporter homologues differently expressed in the nervous system of Drosophila melanogaster
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DOI：
10.1016/s0014-5793(98)01695-0
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发表时间：
1999-01-25
期刊：
影响因子：
3.5
通讯作者：
Birman, S
中科院分区：
文献类型：
--
作者：
Besson, MT;Soustelle, L;Birman, S
在脊椎动物中，兴奋性氨基酸转运蛋白（EAAT）被认为介导兴奋性突触释放的谷氨酸的清除，并维持这种神经递质的细胞外浓度低于兴奋性毒性水平。谷氨酸在昆虫中也被用作神经肌肉接头处的兴奋性神经递质，并且可能在中枢神经系统中，其作用仍有待确定。我们报告了两个果蝇cDNA的分子特征和发育表达模式：dEAAT 1，最近被鉴定为高亲和力谷氨酸转运蛋白[1]，和dEAAT 2，一种与dEAAT 1和哺乳动物EAAT蛋白家族具有很强同源性的新蛋白。通过北方印迹分析和整体原位杂交比较了两个果蝇EAAT基因的发育表达模式。这两种转运蛋白在神经系统的不同细胞类型中转录，并在成人视觉系统中强烈表达。(C)1999年欧洲生物化学学会联合会。
In vertebrates, excitatory amino acid transporters (EAATs) are believed to mediate the removal of glutamate released at excitatory synapses and to maintain extracellular concentrations of this neurotransmitter below excitotoxic levels. Glutamate is also used in insects as an excitatory neurotransmitter at the neuromuscular junction and probably in the central nervous system where its role remains to be established. We report the molecular characterization and developmental expression pattern of two Drosophila cDNAs: dEAAT1, which has recently been identified as a high affinity glutamate transporter [1], and dEAAT2, a novel protein sharing strong homology to dEAAT1 and to the mammalian EAAT protein family. The developmental expression pattern of the two Drosophila EAAT genes has been compared by Northern blot analysis and whole-mount in situ hybridizations. The two transporters are transcribed in distinct cell types of the nervous system and are strongly expressed in the adult visual system. (C) 1999 Federation of European Biochemical Societies.